Tumor necrosis factor (TNF)‐related apoptosis‐inducing ligand (TRAIL) belongs to the TNF cytokine superfamily that specifically induces apoptosis in a broad spectrum of human cancer cell lines but not in most healthy cells. The antitumor potential of recombinant human TRAIL (rhTRAIL) has attracted great attention among biologists and oncologists. However, attempts to express rhTRAIL in Escherichia coli often results in limited yield of bioactive protein due to the formation of inclusion bodies (IBs), which are dense insoluble particulate protein aggregates inside cells. We describe herein a highly simplified method to produce pure bioactive rhTRAIL using E. coli. The method is straightforward and requires only basic laboratory equipment, with highly efficient purification and high yield of renaturation, and may also be applied to produce other proteins that form IBs in E. coli.

中文翻译：

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一种高效纯化和重折叠重组人 TRAIL 的简化方法。

肿瘤坏死因子 (TNF) 相关的凋亡诱导配体 (TRAIL) 属于 TNF 细胞因子超家族，可特异性诱导广谱人类癌细胞系而非大多数健康细胞的凋亡。重组人TRAIL（rhTRAIL）的抗肿瘤潜力引起了生物学家和肿瘤学家的极大关注。然而，由于包涵体 (IB) 的形成，在大肠杆菌中表达 rhTRAIL 的尝试通常会导致生物活性蛋白的产量有限，IBs 是细胞内致密的不溶性颗粒蛋白聚集体。我们在此描述了一种使用大肠杆菌生产纯生物活性 rhTRAIL 的高度简化方法. 该方法简单明了，只需要基本的实验室设备，具有高效纯化和高复性收率，也可用于生产在大肠杆菌中形成 IBs 的其他蛋白质。