The effects of vaginal secretion, its antibacterial peptide fraction, albumin, and bacterial metabolites on the spermatozoon membranes were studied. Vaginal secretion was collected from healthy women; the fraction of antibacterial peptides was isolated with the use of membrane filter; electrophoretically pure BSA served as albumin; bacterial metabolites were isolated from supernatants of cultured Candida albicans yeast and E. coli and Staphylococcus aureus bacteria. Antibacterial activities of the preparations were evaluated by spectrophotometry and microscopy. Albumin in a concentration close to its serum concentration (50 mg/ml) destroyed spermatozoon membranes by 83.8±2.7% with the formation of vesicular debris. The native vaginal secretion and its antibacterial peptide fraction also destroyed spermatozoa by 50.8±4.4 and 18.0±9.7%, respectively. Activities of bacterial metabolites in concentrations close to the natural did not surpass 3%.

中文翻译：

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精子在阴道培养基中存活的不利体液因素

研究了阴道分泌物、其抗菌肽组分、白蛋白和细菌代谢物对精子膜的影响。阴道分泌物采集自健康女性；使用膜滤器分离抗菌肽部分；电泳纯 BSA 作为白蛋白；从培养的白色念珠菌酵母和大肠杆菌和金黄色葡萄球菌的上清液中分离出细菌代谢物。通过分光光度法和显微镜评估制剂的抗菌活性。浓度接近其血清浓度 (50 mg/ml) 的白蛋白破坏了 83.8±2.7% 的精子膜，并形成了囊泡碎片。天然阴道分泌物及其抗菌肽部分也分别破坏了 50.8±4.4% 和 18.0±9.7% 的精子。