We studied changes in the blood cytokine profile of rats 3 h, 1 day, and 8 days after acute stress on the model of 24-h immobilization followed by LPS administration (100 μg/kg intraperitoneally). The concentration of proinflammatory cytokines (particularly of IL-1β and TNFα) significantly decreased at the early stage after stress exposure and physiological saline injection, but increased in the follow-up period and practically did not differ or even surpassed the control level by the end of observations. Under these conditions, the blood content of anti-inflammatory cytokine IL-10 increased most significantly on day 1 of the post-stress period. Restraint stress followed by LPS administration was accompanied by a decrease in the level of proinflammatory cytokines at the early (IFNγ and TNFα) and late stages (IL-1β) of the experiment. Directed modulation of the immune status in animals after acute stress was followed by a significant increase in the content of IL-10 on days 1 and 8, as well as by a tendency toward elevation of IL-4 concentration by the end of the study. The directionality and degree of changes in the cytokine profile of mammalian tissues depend on the type of extreme exposure, duration of the post-stress period, and specific effects of exogenous pathogenic factors in the whole body.

中文翻译：

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单次长期应激暴露后抗原治疗期间大鼠的血细胞因子浓度

我们研究了大鼠在急性应激后 3 小时、1 天和 8 天血液细胞因子谱的变化，该模型采用 24 小时固定，然后是 LPS 给药（100 μg/kg 腹膜内）。应激和生理盐水注射后早期促炎细胞因子（特别是IL-1β和TNFα）浓度显着下降，但在随访期间增加，到最后几乎没有差异甚至超过对照水平的观察。在这些条件下，抗炎细胞因子 IL-10 的血液含量在压力后的第 1 天增加最显着。LPS 给药后的约束压力伴随着实验早期（IFNγ 和 TNFα）和晚期（IL-1β）促炎细胞因子水平的降低。在急性应激后直接调节动物的免疫状态后，第 1 天和第 8 天 IL-10 的含量显着增加，并且在研究结束时 IL-4 浓度有升高的趋势。哺乳动物组织细胞因子谱变化的方向性和程度取决于极端暴露的类型、后应激期的持续时间以及外源性致病因素对全身的具体作用。