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Proliferative Activity of Mouse Splenocytes in Physiological Pregnancy and in Models of Spontaneous and Muramylpeptide-Dependent Abortions
Bulletin of Experimental Biology and Medicine ( IF 0.7 ) Pub Date : 2020-04-01 , DOI: 10.1007/s10517-020-04796-8
K A Artem'eva 1 , M N Boltovskaya 1 , I M Bogdanova 1 , S S Obernikhin 1 , I I Stepanova 1 , A A Stepanov 1 , O A Svitich 2 , O V Kalyuzhin 3
Affiliation  

Spontaneous proliferative activity of splenocytes in female CBA mice and the response of these cells to antigens of allogeneic male BALB/c and DBA/2 mice in a mixed splenocyte culture were evaluated by 3 H-thymidine incorporation in different pregnancy models. ♀CBA×♂BALB/c mating was used for modeling physiological pregnancy. Spontaneous abortions were reproduced by abortion-prone ♀CBA×♂DBA/2 mating. In order to simulate immunostimulant-induced and immunostimulant-potentiated abortions, 0.83 mg/kg muramyl dipeptide β-heptylglycoside was intraperitoneally injected to CBA females mated with BALB/c or DBA/2 males, respectively, on gestation days 5 and 7. The increase in the rate of embryo resorption in the models of spontaneous, induced, and potentiated abortions occurred against the background of an increase in the level of spontaneous proliferation of splenocytes and a decrease in their reactivity to paternal antigens on gestation day 9.

中文翻译:

小鼠脾细胞在生理妊娠和自发性和胞壁肽依赖性流产模型中的增殖活性

雌性 CBA 小鼠脾细胞的自发增殖活性以及这些细胞对混合脾细胞培养物中同种异体雄性 BALB/c 和 DBA/2 小鼠抗原的反应是通过在不同妊娠模型中掺入 3 H-胸苷来评估的。♀CBA×♂BALB/c交配用于模拟生理妊娠。自然流产通过有流产倾向的♀CBA×♂DBA/2交配繁殖。为了模拟免疫刺激剂诱导和免疫刺激剂增强的流产,在妊娠第 5 天和第 7 天,分别向与 BALB/c 或 DBA/2 雄性交配的 CBA 雌性小鼠腹膜内注射 0.83 mg/kg 壁酰二肽 β-庚基糖苷。在自发、诱导、
更新日期:2020-04-01
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