Improved technology for the bioenergetic profiling of human blood cells enables population-based screening for alterations in mitochondrial respiration. Mitochondria are sensitive to oxidative stress, and the aim of this study was to quantify mitochondrial respiration in freshly isolated lymphocytes and monocytes challenged with a bolus of H₂O₂. Mitochondrial reserve capacity, calculated as the difference between basal oxygen consumption and maximal activity after uncoupling of the electron transport chain, was the most sensitive to H₂O₂. Treatment of lymphocytes with 20 μM H₂O₂ reduced the reserve capacity by approximately 50%, while monocyte reserve capacity was five times more resistant. Healthy donors of a similar age were tested to determine the variation between individuals, and within the same individuals tested on several different occasions. Lymphocytes obtained from a population of people aged 70–80 years showed a similar inhibition upon challenge with H₂O₂ as those aged 18–25 years, indicating no decline in resilience with age.

改进的人类血细胞生物能谱分析技术使基于人群的线粒体呼吸变化筛查成为可能。线粒体对氧化应激敏感，因此本研究的目的是量化在大剂量H ₂ O ₂刺激下新鲜分离的淋巴细胞和单核细胞中的线粒体呼吸。线粒体储备容量是根据H ₂ O ₂最敏感的，它是根据基础耗氧量与电子传输链解偶联后的最大活性之间的差异计算得出的。用20μMH ₂ O ₂处理淋巴细胞降低了约50％的储备能力，而单核细胞的储备能力则提高了五倍。对相似年龄的健康供体进行了测试，以确定个体之间的差异，并在相同的个体内进行了几次不同的测试。从70-80岁的人群中获得的淋巴细胞在受到H ₂ O ₂攻击时表现出与18-25岁的人群相似的抑制作用，表明其抗逆能力并未随年龄的增长而下降。