Quantitative phase imaging (QPI) technique is used to determine various biophysical parameters, such as refractive index, cell thickness, morphology, etc. On the other hand, fluorescence microscopy is used to acquire information regarding molecular specificity of the biological cells and tissues. Conventionally, a fully coherent light source such as laser is used in QPI technique to obtain the interference fringes with ease; however, its high coherence is also responsible for the generation of speckle and spurious fringes, which results in degraded image quality and affects the phase measurement results too. In this paper, we report a multi-modal system that can be effectively utilized to acquire time varied diverse information about the biological specimen with high spatial phase sensitivity. Herein, a single unit comprising of a fluorescence microscope and the Linnik based interferometer specially equipped with a partially spatially coherent light source illumination was developed. The integrated system is capable to procure molecular specificity and phase information of biological specimen, in a single shot, utilizing a single-chip color CCD camera. Here, we performed experiments on MG63 osteosarcoma cells, and the composite interferometric-fluorescence images were obtained and then digitally decomposed into red and green colors; and, the phase maps were reconstructed using the Fourier fringe analysis method. Furthermore, the cultured cells were monitored over a time-span to observe and investigate the time dependent morphological changes along with the quantification of cellular adhesion and spreading. Hence, the proposed system can be utilized to quantify time dependent changes in the cell's morphology and in cell adhesion which can be an indicator for the detection of various range of diseases such as arthritis, cancer, osteoporosis and atherosclerosis.

中文翻译：

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使用部分空间相干光源对MG63骨肉瘤细胞进行同步荧光和定量相成像，以监测随时间的形态变化。

定量相成像（QPI）技术用于确定各种生物物理参数，例如折射率，细胞厚度，形态等。另一方面，荧光显微镜用于获取有关生物细胞和组织的分子特异性的信息。常规地，在QPI技术中使用诸如激光之类的全相干光源来轻松获得干涉条纹。但是，其高相干性也导致斑点和杂散条纹的产生，这导致图像质量下降，并且也影响相位测量结果。在本文中，我们报告了一种多模式系统，该系统可有效地获取有关具有高空间相位敏感性的生物标本的时变多样性信息。在这里 开发了由荧光显微镜和基于Linnik的干涉仪组成的单个单元，该单元特别配备了部分空间相干的光源照明。该集成系统能够利用单芯片彩色CCD相机在单次拍摄中获得生物样本的分子特异性和相位信息。在这里，我们对MG63骨肉瘤细胞进行了实验，获得了复合干涉荧光图像，然后将其数字分解为红色和绿色。然后，使用傅里叶条纹分析方法重建相图。此外，在一定的时间跨度上对培养的细胞进行监测，以观察和研究时间依赖性的形态变化以及细胞粘附和扩散的定量。因此，