Despite menaquinones (MKs)-4 and - 7 being known to have extensive biological activities and applications, less attention has been paid to the other MKs. Thus, to obtain a range of MKs to further explore their pharmacological activities, structure-activity relationships, and applications, a chemical screening method for MK-producing strains was established based on high-performance liquid chromatography-ultraviolet (HPLC-UV) technology. Considering that Bacillus strains have proven to be an important MK-producing bioresource, twenty-nine putative Bacillus isolates previously sought from a fermented soybean sample were used for the validation of the chemical screening method, which ultimately led to the discovery of sixteen MK-producing strains. Among them, Bacillus subtilis DC-1 presented excellent ability to produce MKs. Another, a purchased strain of B. amyloliquefaciens was discovered to be an MK-producing strain. These results indicated this screening method was simple and highly efficient for the discovery of MK-producing strains, especially those producing a range of MK structures.

中文翻译：

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基于HPLC-UV技术的产甲萘醌菌株的化学筛选方法。

尽管已知甲萘醌（MKs）-4和-7具有广泛的生物学活性和应用，但对其他MK的关注却较少。因此，为了获得一系列的MK以进一步探索它们的药理活性，结构-活性关系和应用，基于高效液相色谱-紫外（HPLC-UV）技术建立了MK产生菌株的化学筛选方法。考虑到芽孢杆菌属菌株已被证明是生产MK的重要生物资源，以前从发酵大豆样品中寻找到的29种推定芽孢杆菌分离株用于验证化学筛选方法，最终导致发现了16种MK产生菌株。其中，枯草芽孢杆菌DC-1具有极好的产生MK的能力。另一个，发现购买的解淀粉芽孢杆菌菌株是产生MK的菌株。这些结果表明，该筛选方法对于发现MK产生菌株，特别是产生一系列MK结构的菌株而言是简单且高效的。