CellTrace Violet™ is a commonly used fluorescent dye used with flow cytometry to identify cell proliferation. Activated equine lymphocytes were examined using flow cytometry, microscopy and tritiated thymidine proliferation assays. CellTrace Violet™ was incorporated into the equine lymphocytes effectively. Equine lymphocytes proliferated when activated with pokeweed mitogen, but did not proliferate when previously stained with CellTrace Violet™. Serial dilutions of CellTrace Violet™ did not eliminate the inhibition of activated lymphocytes. Equine lymphocyte viability was greater than 90 % for both stained and unstained cells. Based on these data, CellTrace Violet™ is not recommended for the assessment of lymphocyte proliferation in equine cells. The mechanism of inhibition of equine lymphocyte proliferation by CellTrace Violet™ is unknown.

中文翻译：

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CellTrace Violet™抑制马淋巴细胞增殖。

CellTrace Violet™是一种常用的荧光染料，可用于流式细胞术来鉴定细胞增殖。使用流式细胞仪，显微镜检查和tri化胸腺嘧啶核苷增殖分析检查活化的马淋巴细胞。CellTrace Violet™有效地整合到马淋巴细胞中。当用商陆有丝分裂原激活时，马淋巴细胞增殖，而当以前用CellTrace Violet™染色时，马淋巴细胞不增殖。CellTrace Violet™的系列稀释液不能消除对活化淋巴细胞的抑制作用。对于染色的和未染色的细胞，马的淋巴细胞活力均大于90％。基于这些数据，不建议使用CellTrace Violet™评估马细胞中的淋巴细胞增殖。CellTrace Violet™抑制马淋巴细胞增殖的机制尚不清楚。