当前位置: X-MOL 学术J. Microbiol. Methods › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Validation of a screening method for the detection of colistin-resistant E. coli containing mcr-1 in feral swine feces.
Journal of Microbiological Methods ( IF 2.2 ) Pub Date : 2020-03-14 , DOI: 10.1016/j.mimet.2020.105892
Jeffrey C Chandler 1 , Alan B Franklin 1 , Sarah N Bevins 1 , Kevin T Bentler 1 , Jonas Bonnedahl 2 , Christina A Ahlstrom 3 , Bledar Bisha 4 , Susan A Shriner 1
Affiliation  

A method was developed and validated for the detection of colistin-resistant Escherichia coli containing mcr-1 in the feces of feral swine. Following optimization of an enrichment method using EC broth supplemented with colistin (1 μg/mL) and vancomycin (8 μg/mL), aliquots derived from 100 feral swine fecal samples were spiked with of one of five different mcr-1 positive E. coli strains (between 100 and 104 CFU/g), for a total of 1110 samples tested. Enrichments were then screened using a simple boil-prep and a previously developed real-time PCR assay for mcr-1 detection. The sensitivity of the method was determined in swine feces, with mcr-1 E. coli inocula of 0.1-9.99 CFU/g (n = 340), 10-49.99 CFU/g (n = 170), 50-99 CFU/g (n = 255), 100-149 CFU/g (n = 60), and 200-2200 CFU/g (n = 175), which were detected with 32%, 72%, 88%, 95%, and 98% accuracy, respectively. Uninoculated controls (n = 100) were negative for mcr-1 following enrichment.

中文翻译:

用于检测野猪粪便中含有mcr-1的大肠菌素抗性大肠杆菌的筛选方法的验证。

开发并验证了一种用于检测野猪粪便中含有mcr-1的大肠菌素抗性大肠杆菌的方法。优化使用添加了粘菌素(1μg/ mL)和万古霉素(8μg/ mL)的EC肉汤的富集方法后,将100种野生猪粪便样品中的等分试样掺入5种不同的mcr-1阳性大肠杆菌之一。菌株(介于100和104 CFU / g之间),总共测试了1110个样品。然后使用简单的煮沸制剂和先前开发的用于mcr-1检测的实时PCR分析法筛选富集物。该方法的灵敏度在猪粪中测定,其中mcr-1大肠杆菌接种量为0.1-9.99 CFU / g(n = 340),10-49.99 CFU / g(n = 170),50-99 CFU / g (n = 255),100-149 CFU / g(n = 60)和200-2200 CFU / g(n = 175),它们的检出率分别为32%,72%,88%,95%,和98%的准确性。富集后,未接种的对照(n = 100)对mcr-1呈阴性。
更新日期:2020-03-14
down
wechat
bug