Tartary buckwheat shell is an important by-product of Tartary buckwheat production. Previous studies shown that Tartary buckwheat shells are rich in flavonoids, which are responsible for their antioxidant properties. Due to lack of advanced separation technologies, the purification for Tartary buckwheat shell is still in the laboratory scale, and could not realize the industrialization production. According to the results of static adsorption experiment, AB-8 resin was selected for Tartary buckwheat shell flavonoids (TBSF) adsorption. The adsorption isotherm, resin adsorption thermodynamic and dynamic adsorption parameters were studied. And the adsorption of AB-8 resin for TBSF was determined as an endothermic process. Results of preparative chromatography experiment showed that TBSF could be efficiently purified by AB-8 resin. And the optimal parameters were: feed concentration 25 mg/mL, desorption flow rate 2.5 mL/min. Under these conditions, the TBSF were separated effectively. Results of liquid chromatography-mass spectrometer (LC-MS) indicated that there were seven kinds of flavonoids in Tartary buckwheat shell, which were mainly from the 40 and 60% of ethanol elution. Simulated moving bed (SMB) was applied for TBSF purification the first time in this study. The optimal conditions of SMB were as following: adsorption zone flow rate 7.0 mL/min, contaminant removal zone flow rate 17.9 mL/min, product elution zone flow rate 22.3 mL/min, regeneration zone flow rate 21.5 mL/min, water washing zone flow rate 27.5 mL/min, switching time 1260 S, and the purity and yield of TBSF was 90 ± 0.22% and 85 ± 0.28%, respectively. The IC50 values of α-glucosidase inhibition activities and DPPH scavenging activity of the purified TBSF were 57.09 ± 0.15 and 7.92 ± 0.23 μg/mL, respectively. The constituents of TBSF showed higher α-glucosidase inhibition activities and antioxidant than raw TBSF and rutin. The results suggest that SMB is a proper method for industrial production of TBSF, and SMB could be applied for other natural products purification.

中文翻译：

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苦荞壳中类黄酮的模拟移动床纯化。

苦荞壳是苦荞生产的重要副产品。先前的研究表明，苦荞麦壳中富含类黄酮，这是其抗氧化特性的原因。由于缺乏先进的分离技术，苦荞壳的提纯仍处于实验室规模，无法实现工业化生产。根据静态吸附实验结果，选用AB-8树脂对苦荞壳类黄酮（TBSF）进行吸附。研究了吸附等温线，树脂吸附热力学和动态吸附参数。并确定吸热过程为AB-8树脂对TBSF的吸附。制备色谱实验结果表明，AB-8树脂可有效纯化TBSF。最佳参数为：进料浓度25 mg / mL，解吸流速2.5 mL / min。在这些条件下，TBSF被有效分离。液相色谱-质谱仪（LC-MS）的结果表明，苦荞壳中有7种黄酮类化合物，主要来自40％和60％的乙醇洗脱。这项研究首次将模拟移动床（SMB）用于TBSF纯化。SMB的最佳条件如下：吸附区流速7.0 mL / min，污染物去除区流速17.9 mL / min，产物洗脱区流速22.3 mL / min，再生区流速21.5 mL / min，水洗区流速为27.5 mL / min，切换时间为1260 S，TBSF的纯度和收率分别为90±0.22％和85±0.28％。纯化的TBSF的α-葡萄糖苷酶抑制活性和DPPH清除活性的IC50值分别为57.09±0.15和7.92±0.23μg/ mL。TBSF的成分显示出比原始TBSF和芦丁更高的α-葡萄糖苷酶抑制活性和抗氧化剂。结果表明，SMB是工业生产TBSF的合适方法，SMB可用于其他天然产物的纯化。