Abstract

Orthosiphon stamineus (O.S) is widely consumed for its medidcinal value including anti-inflammatory, anti-infective, and diuretic properties. The present study evaluates the cytoprotective, anti-mutagenic, and anticlastogenic efficacies of standardized extract of Orthosiphon stamineus. Normal liver cell line (WRL68) exposed to hydrogen peroxide and serum-deprived media as insults to evaluate cytoprotective and glutathione activation activities of (Et. O. s). Salmonella typhimurium TA98 and TA100 exposed to different concentrations of (Et. O. s). The influence of Et. O. s on mitotic, replicative indices as well as chromosomal aberration (CA) and sister chromatid exchange (SCE) induced in human peripheral blood lymphocytes by mitomycin C (MMC). The Et. O.s proved to be a potent scavenger for hydrogen peroxide and other free radicals in serum-depraved media, which showed to stimulate glutathione production in liver cells line. Moreover, it did not induce mutations in S. typhimurium subspecies TA98 and TA100. The standardized extract exhibited powerful antimutagenic activities as verified against both 2-nitrofluorene and sodium azide in S. typhimurium TA98 and TA100 cells, respectively. Cytogenetic tests showed high concentrations of Et. O. s to reduce the values of mitotic and replicative indices without any accompanying side effects, such as chromosomal abnormalities or SCE. To ameliorate MMC effects, pretreatment with the extract proofed to be efficient protocol. These data suggests that O. stamineus extract could be useful as cytoprotective, antimutagenic, and anticlastogenic efficacies, which owes to its potent chemoprevention, antioxidant, and glutathione activation properties.

摘要

Orthosiphon stamineus ( OS ) 因其药用价值而被广泛使用，包括抗炎、抗感染和利尿特性。本研究评估了Orthosiphon stamineus标准化提取物的细胞保护、抗诱变和抗碎裂功效。将正常肝细胞系 (WRL68) 暴露于过氧化氢和血清剥夺培养基作为损伤，以评估 (Et. O. s ) 的细胞保护和谷胱甘肽活化活性。鼠伤寒沙门氏菌TA98 和 TA100 暴露于不同浓度的 (Et. O. s )。等的影响。O.s丝裂霉素C (MMC)在人外周血淋巴细胞中诱导的有丝分裂、复制指数以及染色体畸变 (CA) 和姐妹染色单体交换 (SCE )。等。Os被证明是一种有效的清除血清过氧化氢和其他自由基的清除剂，这表明它可以刺激肝细胞系中谷胱甘肽的产生。此外，它不会在鼠伤寒沙门氏菌亚种 TA98 和 TA100 中诱导突变。标准化提取物在鼠伤寒沙门氏菌TA98 和 TA100 细胞中分别对 2-硝基芴和叠氮化钠具有强大的抗诱变活性。细胞遗传学测试显示高浓度的 Et。O.s降低有丝分裂和复制指数的值，而不会产生任何伴随的副作用，例如染色体异常或 SCE。为了改善 MMC 效果，用提取物进行预处理证明是有效的方案。这些数据表明，O. stamineus提取物可用作细胞保护、抗诱变和抗碎裂的功效，这归功于其有效的化学预防、抗氧化和谷胱甘肽活化特性。