Aqueous two-phase extraction of wedelolactone from Eclipta alba was studied using the polymer-salt system. The system consisted of polyethylene glycol (PEG) as a top phase (polymer) and sodium citrate as a bottom phase (salt). Process parameters such as PEG concentration, PEG molecular weight, salt concentration, and pH have been optimized using response surface methodology (RSM) with the help of central composite design (CCD). The optimized conditions for aqueous two-phase system (ATPS), in the case of one factor at a time approach, were found as PEG 6000, PEG concentration 18% (w/v), salt concentration 16% (w/v), and pH 7; with maximum extraction yield of 6.52 mg/g. While, RSM studies showed maximum extraction yield of 6.73 mg/g with the optimized parameters as PEG 6000, PEG concentration 18% (w/v), salt concentration 17.96% (w/v), and pH 7. ATPS was found to give a 1.3 fold increase in the extraction yield of wedelolactone as compared to other conventional extraction methods.

使用聚合物-盐体系研究了从白墨旱草中水松香内酯的两相水相萃取。该系统由作为上层相（聚合物）的聚乙二醇（PEG）和作为下层相（盐）的柠檬酸钠组成。借助中央复合设计（CCD），使用响应表面方法（RSM）对诸如PEG浓度，PEG分子量，盐浓度和pH值等工艺参数进行了优化。在一次因素的情况下，发现水相两相系统（ATPS）的最佳条件为PEG 6000，PEG浓度为18％（w / v），盐浓度为16％（w / v），pH值为7；最大提取量为6.52 mg / g。同时，RSM研究显示，最佳提取参数为PEG 6000，PEG浓度为18％（w / v），盐浓度为17.96％（w / v）和pH为7时，最大提取量为6.73 mg / g 。与其他常规提取方法相比，韦德内酯的提取率提高了1.3倍。