A Gram-negative, aerobic, motile, non-spore-forming and rod-shaped bacterium, designated strain B18-69 T , was isolated from oil-well production liquid in Baolige oilfield, China. The strain was able to grow at pH 6–9.5 (optimum at pH 7), in 0–4% (w/v) NaCl (optimum at 0.5–1%, w/v) and at 35–60 °C (optimum at 55 °C). Major cellular fatty acids were C 16:0 , C 19:0 cyclo ω8c, C 17:0 cyclo and C 18:1 ω7c. The predominant respiratory quinone was ubiquinone 8. Major polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG) and phosphatidylcholine (PC). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain B18-69 T was most closely related to Tepidiphilus margaritifer DSM 15129 T (98.8% similarity). The draft genome of strain B18-69 T was composed of 2,250,419 bp, and the G+C content was 64.6 mol%. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain B18-69 T and T. margaritifer DSM 15129 T were 90.9% and 68.9%, respectively. Genotypic and phenotypic features indicate that strain B18-69 T represents a novel species of the genus Tepidiphilus , for which the name Tepidiphilus baoligensis sp. nov. is proposed. The type strain is B18-69 T (= CGMCC 1.13573 T = KCTC 62782 T ).

中文翻译：

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Tepidiphilus baoligensis sp. 11 月，从油藏中分离出的嗜氢科新细菌

从宝利格油田油井生产液中分离到一株革兰氏阴性、需氧、能动、不产孢子的杆状细菌，定名为B18-69 T。该菌株能够在 pH 6–9.5（最佳 pH 7）、0–4% (w/v) NaCl（最佳 0.5–1%，w/v）和 35–60 °C（最佳在 55°C）。主要的细胞脂肪酸是 C 16:0、C 19:0 环 ω8c、C 17:0 环和 C 18:1 ω7c。主要的呼吸醌是泛醌 8。主要的极性脂质是磷脂酰乙醇胺 (PE)、磷脂酰甘油 (PG)、二磷脂酰甘油 (DPG) 和磷脂酰胆碱 (PC)。基于 16S rRNA 基因序列的系统发育分析表明，菌株 B18-69 T 与 Tepidiphilus margaritifer DSM 15129 T 的亲缘关系最密切（相似性为 98.8%）。菌株 B18-69 T 的基因组草图由 2,250,419 bp 组成，G+C 含量为 64.6 mol%。菌株 B18-69 T 和 T. margaritifer DSM 15129 T 之间的平均核苷酸同一性 (ANI) 和数字 DNA-DNA 杂交 (dDDH) 值分别为 90.9% 和 68.9%。基因型和表型特征表明菌株 B18-69 T 代表了 Tepidiphilus 属的一个新物种，其名称为 Tepidiphilus baoligensis sp。十一月 被提议。类型菌株是 B18-69 T (= CGMCC 1.13573 T = KCTC 62782 T)。