Singlet oxygen (1O2) is widely regarded as the main cytotoxic substance that induces the biological damage for photodynamic therapy (PDT). In this study, the previously developed near‐infrared (NIR) optical imaging system was optimized for fast imaging of 1O2 luminescence. The optical imaging system enables direct imaging of 1O2 luminescence in blood vessels within 2 s during vascular‐targeted PDT (V‐PDT), which makes this system extremely practical for in vivo studies. The dependence of RB concentration on 1O2 luminescence image was investigated for V‐PDT, and the data imply that 1270 nm signal is attributed to 1O2 luminescence. The imaging system operates with a field of view of 9.60 × 7.68 mm2 and a spatial resolution of 30 μm, which holds the potential to elucidate the correlation between cumulative 1O2 luminescence and vasoconstriction for V‐PDT.

中文翻译：

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血管靶向光动力治疗过程中血管中的单线态氧发光图像

单线态氧 (1O2) 被广泛认为是诱导光动力疗法 (PDT) 生物损伤的主要细胞毒性物质。在这项研究中，之前开发的近红外 (NIR) 光学成像系统针对 1O2 发光的快速成像进行了优化。该光学成像系统能够在血管靶向 PDT (V-PDT) 期间在 2 秒内对血管中的 1O2 发光进行直接成像，这使得该系统对于体内研究非常实用。研究了 V-PDT 的 RB 浓度对 1O2 发光图像的依赖性，数据表明 1270 nm 信号归因于 1O2 发光。成像系统的工作视场为 9.60 × 7.68 mm2，空间分辨率为 30 μm，