Site‐specific recombinase enzymes function in heterologous cellular environments to initiate strand‐switching reactions between unique DNA sequences termed recombinase binding sites. Depending on binding site position and orientation, reactions result in integrations, excisions, or inversions of targeted DNA sequences in a precise and predictable manner. Here, we established five different stable recombinase expression lines in maize through Agrobacterium‐mediated transformation of T‐DNA molecules that contain coding sequences for Cre, R, FLPe, phiC31 Integrase, and phiC31 excisionase. Through the bombardment of recombinase activated DsRed transient expression constructs, we have determined that all five recombinases are functional in maize plants. These recombinase expression lines could be utilized for a variety of genetic engineering applications, including selectable marker removal, targeted transgene integration into predetermined locations, and gene stacking.

中文翻译：

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玉米中的位点特异性重组酶基因组工程工具包。

位点特异性重组酶在异源细胞环境中起作用，以启动称为重组酶结合位点的独特DNA序列之间的链转换反应。根据结合位点的位置和方向，反应会以精确和可预测的方式导致目标DNA序列的整合，切除或倒置。在这里，我们通过农杆菌在玉米中建立了五个不同的稳定重组酶表达系介导的T-DNA分子转化，其中包含Cre，R，FLPe，phiC31整合酶和phiC31切除酶的编码序列。通过轰击重组酶激活的DsRed瞬时表达构建体，我们确定了所有五个重组酶在玉米植物中均具有功能。这些重组酶表达系可用于多种基因工程应用，包括选择性标记去除，靶向转基因整合到预定位置以及基因堆叠。