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Metagenomics for taxonomy profiling: tools and approaches.
Bioengineered ( IF 4.9 ) Pub Date : 2020-12-01 , DOI: 10.1080/21655979.2020.1736238
Mukesh Kumar Awasthi 1, 2 , B Ravindran 3 , Surendra Sarsaiya 4 , Hongyu Chen 5 , Steven Wainaina 2 , Ekta Singh 6 , Tao Liu 1 , Sunil Kumar 6 , Ashok Pandey 7 , Lal Singh 6 , Zengqiang Zhang 1
Affiliation  

The study of metagenomics is an emerging field that identifies the total genetic materials in an organism along with the set of all genetic materials like deoxyribonucleic acid and ribose nucleic acid, which play a key role with the maintenance of cellular functions. The best part of this technology is that it gives more flexibility to environmental microbiologists to instantly pioneer the immense genetic variability of microbial communities. However, it is intensively complex to identify the suitable sequencing measures of any specific gene that can exclusively indicate the involvement of microbial metagenomes and be able to advance valuable results about these communities. This review provides an overview of the metagenomic advancement that has been advantageous for aggregation of more knowledge about specific genes, microbial communities and its metabolic pathways. More specific drawbacks of metagenomes technology mainly depend on sequence-based analysis. Therefore, this 'targeted based metagenomics' approach will give comprehensive knowledge about the ecological, evolutionary and functional sequence of significantly important genes that naturally exist in living beings either human, animal and microorganisms from distinctive ecosystems.

中文翻译:

用于分类分析的宏基因组学:工具和方法。

宏基因组学研究是一个新兴领域,它识别生物体中的全部遗传物质以及所有遗传物质的集合,如脱氧核糖核酸和核糖核酸,它们对维持细胞功能起着关键作用。这项技术最好的部分是它为环境微生物学家提供了更大的灵活性,可以立即开创微生物群落的巨大遗传变异性。然而,要确定任何特定基因的合适测序措施是非常复杂的,这些基因可以专门表明微生物宏基因组的参与,并能够推进关于这些群落的有价值的结果。这篇综述概述了宏基因组的进步,这有利于聚集更多关于特定基因的知识,微生物群落及其代谢途径。宏基因组技术更具体的缺点主要取决于基于序列的分析。因此,这种“基于靶向的宏基因组学”方法将全面了解自然存在于人类、动物和来自独特生态系统的微生物中的重要基因的生态、进化和功能序列。
更新日期:2020-05-06
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