The present study aimed to investigate the effect of a compressive force (CF) on the expression of receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG), and vascular endothelial growth factor (VEGF) in murine osteocytes (MLO-Y4) as well as animal study. After application of a CF for 1, 3, 6, and 12 h, gene and protein expression of RANKL, OPG, and VEGF in MLO-Y4 cells were determined by real-time PCR and enzyme-linked immunosorbent assay (ELISA). Furthermore, the effect of a stretch-activated (S-A) channel was examined by gadolinium (Gd3+) administration. In an animal experiment, the expression of these factors in osteocytes of alveolar bone was examined after experimental tooth movement in rats. After CF application, significant increases in RANKL, VEGF and RANKL/OPG ratio were shown. The upregulated gene and protein levels of these factors were reduced by Gd3+ administration. After tooth movement, upregulated RANKL and VEGF were imunohistochemically shown in osteocytes of alveolar bone. These findings suggest that CF application on osteocytes elevates expression of osteoclast-inducing factor and angiogenesis factor in vivo and vitro.

中文翻译：

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连续压缩力对骨细胞中RANKL，OPG和VEGF表达的影响。

本研究旨在研究压缩力（CF）对鼠骨细胞（MLO）中核因子kappa-B配体（RANKL），骨保护素（OPG）和血管内皮生长因子（VEGF）受体激活剂表达的影响-Y4）以及动物研究。在CF分别作用1、3、6和12小时后，通过实时PCR和酶联免疫吸附测定（ELISA）确定MLO-Y4细胞中RANKL，OPG和VEGF的基因和蛋白表达。此外，通过g（Gd3 +）施用检查了拉伸激活（SA）通道的作用。在动物实验中，在大鼠进行实验性牙齿移动后，检查了这些因子在牙槽骨骨细胞中的表达。CF施用后，RANKL，VEGF和RANKL / OPG比例显着增加。通过施用Gd3 +可以降低这些因子的上调基因和蛋白质水平。牙齿运动后，在牙槽骨的骨细胞中免疫组织化学显示了RANKL和VEGF的上调。这些发现表明CF在骨细胞上的应用在体内和体外提高了破骨细胞诱导因子和血管生成因子的表达。