Chondrocytes are utilized to cartilage regeneration by being harvested through enzymatic digestion and expanded by monolayer culture. However, these procedures will cause deterioration and dedifferentiation of the chondrocytes. In addition, scaffolds are often needed to provide the cartilage with mechanical strength and three-dimensional structures. We tried to use diced cartilage prepared using a micro-slicer without digestion, monolayer culture or scaffolds. In this study, an appropriate culture condition to induce the fusion of diced cartilage in vitro and cartilage regeneration in vitro and in vivo was determined to realize a scaffold-free cartilage regeneration. As a result, diced cartilages aggregated when they were cultured more than 5 weeks in the media containing 10% fetal bovine serum (FBS). Diced cartilage cultured for 7 weeks with the media containing 10%, followed by the culture with the media containing insulin-like growth factor-1 for 5 weeks in the ultralow attachment plate showed most prominent cartilage formation both in vitro and in vivo. The volume of regenerated cartilage was 2.14 times larger than that of the original cartilage. These results indicated that large regenerative cartilage from a small amount of cartilage was achieved without deterioration or dedifferentiation.

中文翻译：

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建立用于制备再生软骨的微型切片器的新技术，以制备三维切成丁的软骨。

软骨细胞通过酶消化收集并通过单层培养扩增，从而用于软骨再生。但是，这些步骤将导致软骨细胞的恶化和去分化。另外，经常需要支架来提供具有机械强度和三维结构的软骨。我们尝试使用通过微型切片机制备的未切割，未消化，单层培养或支架的软骨。在这项研究中，确定合适的培养条件以诱导切成丁的软骨在体外融合以及在体外和体内软骨再生，以实现无支架软骨再生。结果，在含有10％胎牛血清（FBS）的培养基中培养5周以上时，切成小方块的软骨会聚集。用含10％的培养基将切成丁的软骨培养7周，然后在超低附着平板中用含胰岛素样生长因子-1的培养基培养5周，在体外和体内均显示出最突出的软骨形成。再生软骨的体积是原始软骨的2.14倍。这些结果表明，从少量软骨获得了大的再生软骨，而没有变质或去分化。