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Stereological study of the placental structure in abortion-prone mice model (CBA/J×DBA/2J).
Annals of Anatomy ( IF 2.2 ) Pub Date : 2020-03-12 , DOI: 10.1016/j.aanat.2020.151508 Mahsa Sadat Hosseini 1 , Mohammad Ali-Hassanzadeh 2 , Elham Nadimi 3 , Saied Karbalay-Doust 4 , Ali Noorafshan 4 , Behrouz Gharesi-Fard 5
Annals of Anatomy ( IF 2.2 ) Pub Date : 2020-03-12 , DOI: 10.1016/j.aanat.2020.151508 Mahsa Sadat Hosseini 1 , Mohammad Ali-Hassanzadeh 2 , Elham Nadimi 3 , Saied Karbalay-Doust 4 , Ali Noorafshan 4 , Behrouz Gharesi-Fard 5
Affiliation
Recurrent spontaneous abortion (RSA) is an important reproductive health issue defined as the loss of two or more consecutive pregnancies before the 20th week of gestation, affecting 2-5% of couples. This study aimed to evaluate the volume, number of cells, and length of the vessels in the placenta in normal and abortion-prone (AP) pregnant mice on gestational day (gd) 13.5. Fetal and placental tissues of female CBA/J mated DBA/2J (AP group) and BALB/c (normal pregnant group) were collected and prepared for stereological assessments on gd13.5. The volumes of the placenta and its main layers decidua basalis (Db), junctional zone (Jz), and labyrinth zone (Lz) were investigated. The number of spongiotrophoblast cells, glycogen cells, giant cells, trophoblast cells, lymphocytes, and neutrophils were estimated as well. The AP group showed a reduction in the volume of the placenta (48.7%) and its components. Moreover, the number of spongiotrophoblast cells (66.7%), glycogen cells (76.2%), giant cells (73.3%), and trophoblast cells (81.4%) was decreased in AP compared to normal pregnant (NP) mice. Also, in AP group recognized a 10-fold increase in the number of lymphocytes and a four-fold increase in the number of neutrophils in comparison to the NP group (p < 0.05). Activation of different immune cell types might induce systemic inflammation at the feto-maternal interface, resulting in impaired placenta formation and abortion.
中文翻译:
易流产小鼠模型(CBA / J×DBA / 2J)胎盘结构的立体学研究。
反复自然流产(RSA)是一个重要的生殖健康问题,定义为在妊娠第20周之前两次或两次以上连续怀孕的流产,影响了2至5对夫妇。这项研究旨在评估正常和易流产(AP)妊娠小鼠在妊娠日(gd)13.5时胎盘中血管的数量,细胞数量和长度。收集雌性CBA / J交配的DBA / 2J(AP组)和BALB / c(正常妊娠组)的胎儿和胎盘组织,并对其进行gd13.5的体格评估。研究了胎盘及其主要层的蜕膜基础(Db),交界区(Jz)和迷宫区(Lz)的体积。还估计了海绵滋养细胞,糖原细胞,巨细胞,滋养细胞,淋巴细胞和嗜中性粒细胞的数量。AP组显示胎盘及其成分的体积减少了(48.7%)。此外,与正常怀孕(NP)小鼠相比,AP的海绵滋养细胞(66.7%),糖原细胞(76.2%),巨细胞(73.3%)和滋养细胞(81.4%)数量减少。同样,在AP组中,与NP组相比,淋巴细胞数量增加了10倍,嗜中性粒细胞数量增加了4倍(p <0.05)。不同免疫细胞类型的激活可能会在胎儿-母亲界面处引起全身性炎症,导致胎盘形成和流产受损。与正常怀孕(NP)小鼠相比,AP的患病率降低了4%。同样,在AP组中,与NP组相比,淋巴细胞数量增加了10倍,嗜中性粒细胞数量增加了4倍(p <0.05)。不同免疫细胞类型的激活可能会在胎儿-母亲界面处引起全身性炎症,导致胎盘形成和流产受损。与正常怀孕(NP)小鼠相比,AP的患病率降低了4%。同样,在AP组中,与NP组相比,淋巴细胞数量增加了10倍,嗜中性粒细胞数量增加了4倍(p <0.05)。不同免疫细胞类型的激活可能会在胎儿-母亲界面处引起全身性炎症,导致胎盘形成和流产受损。
更新日期:2020-03-12
中文翻译:
易流产小鼠模型(CBA / J×DBA / 2J)胎盘结构的立体学研究。
反复自然流产(RSA)是一个重要的生殖健康问题,定义为在妊娠第20周之前两次或两次以上连续怀孕的流产,影响了2至5对夫妇。这项研究旨在评估正常和易流产(AP)妊娠小鼠在妊娠日(gd)13.5时胎盘中血管的数量,细胞数量和长度。收集雌性CBA / J交配的DBA / 2J(AP组)和BALB / c(正常妊娠组)的胎儿和胎盘组织,并对其进行gd13.5的体格评估。研究了胎盘及其主要层的蜕膜基础(Db),交界区(Jz)和迷宫区(Lz)的体积。还估计了海绵滋养细胞,糖原细胞,巨细胞,滋养细胞,淋巴细胞和嗜中性粒细胞的数量。AP组显示胎盘及其成分的体积减少了(48.7%)。此外,与正常怀孕(NP)小鼠相比,AP的海绵滋养细胞(66.7%),糖原细胞(76.2%),巨细胞(73.3%)和滋养细胞(81.4%)数量减少。同样,在AP组中,与NP组相比,淋巴细胞数量增加了10倍,嗜中性粒细胞数量增加了4倍(p <0.05)。不同免疫细胞类型的激活可能会在胎儿-母亲界面处引起全身性炎症,导致胎盘形成和流产受损。与正常怀孕(NP)小鼠相比,AP的患病率降低了4%。同样,在AP组中,与NP组相比,淋巴细胞数量增加了10倍,嗜中性粒细胞数量增加了4倍(p <0.05)。不同免疫细胞类型的激活可能会在胎儿-母亲界面处引起全身性炎症,导致胎盘形成和流产受损。与正常怀孕(NP)小鼠相比,AP的患病率降低了4%。同样,在AP组中,与NP组相比,淋巴细胞数量增加了10倍,嗜中性粒细胞数量增加了4倍(p <0.05)。不同免疫细胞类型的激活可能会在胎儿-母亲界面处引起全身性炎症,导致胎盘形成和流产受损。