The interaction between adipocytes and macrophages in obese tissues plays a critical role in the onset of metabolic syndromes. This study aimed to evaluate the modulatory effect of diosmetin on anti‐inflammatory and anti‐lipolytic activities in the coculture of macrophages and adipocytes. The secretion of inflammatory mediators increased in a coculture medium, however, diosmetin significantly reduced the levels of these inflammatory mediators such as nitric oxide (NO), tumor necrosis factor‐α, and monocyte chemoattractant protein. Diosmetin down‐regulated the protein expression of inducible NO synthase in cocultured macrophages and adipocytes, and inhibited the phosphorylation of mitogen‐activated protein kinases and the translocation of p65 and p50 to the nucleus. Moreover, it suppressed the phosphorylation of hormone‐sensitive lipase and the production of fatty acid‐binding protein 4, and increased the mRNA expression of adiponectin in cocultured adipocytes by 18%–35%. These results indicate that diosmetin inhibited inflammation and lipolysis in the crosstalk between adipocytes and macrophages; diosmetin‐containing foods could be used in dietary therapy for the prevention of obesity‐related metabolic syndromes.

中文翻译：

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薯met皂素对脂肪细胞和巨噬细胞共培养的炎症和脂解的抑制作用。

肥胖组织中脂肪细胞与巨噬细胞之间的相互作用在代谢综合征的发作中起着至关重要的作用。这项研究旨在评估薯os皂素对巨噬细胞和脂肪细胞共培养中抗炎和抗脂解活性的调节作用。共培养液中炎性介质的分泌增加，但是，薯os皂素显着降低了这些炎性介质的水平，例如一氧化氮（NO），肿瘤坏死因子-α和单核细胞趋化蛋白。Diosmetin下调了共培养的巨噬细胞和脂肪细胞中可诱导的NO合酶的蛋白表达，并抑制了促分裂原活化蛋白激酶的磷酸化以及p65和p50向核的转运。此外，它抑制了激素敏感性脂肪酶的磷酸化和脂肪酸结合蛋白4的产生，并使脂联素在共培养的脂肪细胞中的mRNA表达增加了18％至35％。这些结果表明，薯os皂素抑制了脂肪细胞和巨噬细胞之间的串扰中的炎症和脂解。含薯os皂素的食物可用于饮食疗法，以预防肥胖相关的代谢综合症。