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Impact of a novel Rickettsia symbiont on the life history and virus transmission capacity of its host whitefly ( Bemisia tabaci )
Insect Science ( IF 4 ) Pub Date : 2020-07-25 , DOI: 10.1111/1744-7917.12797
Teng Lei 1 , Jing Zhao 1 , Hua-Ling Wang 1 , Yin-Quan Liu 1 , Shu-Sheng Liu 1
Affiliation  

Rickettsia consists of some of the most prevalent symbionts of insects and often plays a significant role in the biology of its hosts. Recently, a maternally inherited Torix group Rickettsia, provisionally named as RiTBt, was recorded in a species of notorious pest whitefly, tentatively named as Asia II 1, from the Bemisia tabaci complex. The role of this Rickettsia in the biology of its host is unknown. Here we investigated the impact of RiTBt on the performance and virus transmission capacity of Asia II 1. RiTBt did not significantly affect the life history parameters of the whitefly when the host insect was reared on tobacco, tomato, and cotton, three host plants with relatively low, medium and high suitability to the whitefly. Intriguingly, RiTBt slightly enhanced whitefly transmission of cotton leaf curl Multan virus (CLCuMuV), a virus that is transmitted by the whitefly in the field and has caused extensive damage to cotton production. Specifically, compared with whiteflies without RiTBt, following a 48 h virus acquisition whiteflies with RiTBt had higher titer of virus and showed higher efficiency of virus transmission. A rickettsial secretory protein BtR242 was identified as a putative virus-binding protein, and was observed to interact with the coat protein of CLCuMuV in vitro. Viral infection of the whitefly downregulated gene transcript level of BtR242 gene. These observations indicate that RiTBt has limited impact on the biology of the Asia II 1 whitefly, and whether this symbiont has functions in the biology of other host whiteflies warrants future investigations. This article is protected by copyright. All rights reserved.

中文翻译:

一种新型立克次体共生体对其寄主粉虱(Bemisia tabaci)生活史和病毒传播能力的影响

立克次体由一些最普遍的昆虫共生体组成,通常在其宿主的生物学中发挥重要作用。最近,一种母系遗传的 Torix 组立克次体,暂时命名为 RiTBt,被记录在一种臭名昭著的害虫粉虱中,暂时命名为 Asia II 1,来自烟粉虱复合体。这种立克次体在其宿主生物学中的作用尚不清楚。在这里,我们调查了 RiTBt 对 Asia II 1 性能和病毒传播能力的影响。当寄主昆虫在烟草、番茄和棉花这三种寄主植物上饲养时,RiTBt 对粉虱的生活史参数没有显着影响。对粉虱的低、中和高适应性。有趣的是,RiTBt 略微增强了棉花卷叶木尔坦病毒 (CLCuMuV) 的粉虱传播,一种由粉虱在田间传播的病毒,对棉花生产造成了广泛的破坏。具体来说,与没有 RiTBt 的粉虱相比,在 48 小时病毒采集后,含有 RiTBt 的粉虱具有更高的病毒滴度,并显示出更高的病毒传播效率。立克次体分泌蛋白 BtR242 被鉴定为推定的病毒结合蛋白,并在体外观察到与 CLCuMuV 的外壳蛋白相互作用。粉虱的病毒感染下调 BtR242 基因的基因转录水平。这些观察表明 RiTBt 对 Asia II 1 粉虱的生物学影响有限,这种共生体是否在其他宿主粉虱的生物学中具有功能值得未来研究。本文受版权保护。版权所有。
更新日期:2020-07-25
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