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Leaf mesophyll K+ and Cl− fluxes and reactive oxygen species production predict rice salt tolerance at reproductive stage in greenhouse and field conditions
Plant Growth Regulation ( IF 4.2 ) Pub Date : 2020-05-05 , DOI: 10.1007/s10725-020-00619-y
Miing-Tiem Yong , Celymar Angela Solis , Barkat Rabbi , Samsul Huda , Rong Liu , Meixue Zhou , Lana Shabala , Gayatri Venkataraman , Sergey Shabala , Zhong-Hua Chen

Extensive research on salinity tolerance in rice has been mostly carried out at the seedling stage in single experimental trials. Here, we aimed to understand the roles of ion transport and oxidative responses of leaf mesophyll in salinity tolerance of rice (Oryza sativa L.) at its reproductive stage using comparative investigations in both greenhouse and field trials. Two experimental trials were conducted to assess the salt tolerance of three rice cultivars at their reproductive stage in greenhouse and field. We employed agronomic, physiological, electrophysiological, molecular and cell imaging techniques to compare physiological response of control and salinity stressed rice plants. Salinity had the most severe effect to Koshihikari, followed by Doongara and Reiziq. We found that K+ retention and low recovery Cl− efflux in mesophyll cells confers salt tolerance in rice. Moderate to strong correlations were found between growth parameters and net K+ flux (r2 = 0.45–0.60), and net Cl− flux (r2 = 0.47–0.72). Also, dynamic ROS production and regulation of the NADPH oxidase gene, OsRBOHD, in mesophyll cells is crucial for salt tolerance of rice at the reproductive stage. OsRBOHD expression was significantly correlated to recovery ion fluxes (r2 = 0.45–0.64). This study brings together, for the first time, potential links between cellular ionic stress and oxidative stress components of salinity tolerance in rice at the reproductive stage in both greenhouse and field conditions. Our study will provide guidance to examine crop salinity tolerance at reproductive stages in controlled environments and natural climatic conditions in the future.

中文翻译:

叶片叶肉 K+ 和 Cl- 通量和活性氧产生预测温室和田间条件下水稻生殖阶段的耐盐性

水稻耐盐性的广泛研究大多在苗期进行,单次试验。在这里,我们旨在通过温室和田间试验的比较研究,了解叶片叶肉的离子转运和氧化反应在水稻(Oryza sativa L.)生殖期耐盐性中的作用。进行了两项实验试验,以评估温室和大田中三个水稻品种在生殖阶段的耐盐性。我们采用农艺学、生理学、电生理学、分子和细胞成像技术来比较对照和盐胁迫水稻植株的生理反应。盐度对越光的影响最严重,其次是 Doongara 和 Reiziq。我们发现叶肉细胞中的 K+ 保留和低回收的 Cl- 流出使水稻具有耐盐性。在生长参数和净 K+ 通量 (r2 = 0.45–0.60) 和净 Cl− 通量 (r2 = 0.47–0.72) 之间发现中度至强相关性。此外,叶肉细胞中 NADPH 氧化酶基因 OsRBOHD 的动态 ROS 产生和调节对于水稻在生殖阶段的耐盐性至关重要。OsRBOHD 表达与恢复离子通量显着相关(r2 = 0.45-0.64)。本研究首次将温室和田间条件下水稻育种期耐盐性的细胞离子胁迫和氧化胁迫成分之间的潜在联系结合在一起。
更新日期:2020-05-05
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