Some miRNAs are supposed to play a role in insulin resistance and metabolic disorders. Such miRNAs can be differentially expressed in response to a pharmacologic intervention for insulin resistance as a biomarker/risk factor for insulin resistance. This study aimed at determining the effect of Metformin on miR320 expression in insulin-resistant (IR) adipocytes. The 3T3L1 cells were expanded in DMEM, differentiated into adipocytes by differentiating medium, became resistant to insulin, and then were treated with ascending concentrations of Metformin. Quantitative real-time PCR was performed to profile the miR320 expression in 3T3L1 adipocytes, IR adipocytes, and Metformin-treated IR adipocytes. Compared to the normal adipocytes, IR adipocytes exhibited a significantly higher level of miR320 expression, however, in response to Metformin graded concentrations, IR adipocytes down-regulated miR320 and were almost at normal level. The maximum effect of Metformin was at 10 mM. In IR adipocytes, miR320 expression is over-expressed which can be down-regulated by Metformin treatment. The findings provide some information on a potentially new marker to determine insulin resistance and to predict response to insulin resistance therapy.

中文翻译：

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递增浓度的二甲双胍治疗miR320在胰岛素抵抗的脂肪细胞中的表达下降。

一些miRNA可能在胰岛素抵抗和代谢异常中起作用。可以响应于作为胰岛素抵抗的生物标志物/风险因子的胰岛素抵抗的药理学干预来响应地表达这样的miRNA。这项研究旨在确定二甲双胍对胰岛素抵抗（IR）脂肪细胞中miR320表达的影响。3T3L1细胞在DMEM中扩增，通过分化培养基分化为脂肪细胞，对胰岛素具有抗性，然后用递增浓度的二甲双胍处理。进行实时定量PCR分析3T3L1脂肪细胞，IR脂肪细胞和二甲双胍处理的IR脂肪细胞中miR320表达。与正常脂肪细胞相比，IR脂肪细胞显示出显着更高的miR320表达水平，但是，响应二甲双胍分级浓度，IR脂肪细胞下调miR320，几乎处于正常水平。二甲双胍的最大作用为10 mM。在IR脂肪细胞中，miR320表达过表达，而二甲双胍处理可能下调miR320的表达。这些发现为确定胰岛素抵抗和预测对胰岛素抵抗治疗的反应的潜在新标志物提供了一些信息。