The objective of this work was to determine the risk of exposure to Dirofilaria immitis in the stray dog population and people living in the metropolitan area of Puebla City, Mexico, using serologic methods. A total of 283 blood salmples were collected from dogs and 254 salmples from people. The canine samples were analyzed using a commercial kit to detect D. immitis antigens and the Knott test to detect microfilariae. Human samples were analyzed by ELISAs to detect antibodies against D. immitis and Wolbachia anti-WSP, as well as Western blot to identify specific bands of the parasite in the adult antigenic extract of D. immitis. Positive results were represented on a GIS map that included layers with geoenvironmental information relevant to parasite transmission in the study area. The prevalence of D. immitis in the canine stray population was 2.12 %, with positive cases found in individuals aged 1-10 years. Human seroprevalence was established at 5.11 %, with women and men similarly affected. By age, a significantly higher seroprevalence was observed in younger individuals (<18 years) than in the rest of the population. The geolocation of the results showed that positive cases were found in the environment (within 2 km) of potentially favorable areas for mosquito breeding. The importance of human influence in the creation and maintenance of favorable conditions for the transmission of canine and human dirofilariosis within an urban area is highlighted, both by the lack of environmental sanitation and by the absence of measures to limit or eliminate the canine reservoir population.

中文翻译：

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墨西哥普埃布拉州犬和人铁丝虫病的患病率。

这项工作的目的是使用血清学方法确定墨西哥流浪狗人群和墨西哥普埃布拉市都会区居民暴露于铁丝虫病的风险。从狗中总共采集了283个血样，从人身上采集了254个血样。使用市售试剂盒分析犬样本以检测D. immitis抗原，并使用诺氏检验法检测微丝aria。通过ELISA分析人体样品，以检测针对D. immitis和Wolbachia抗WSP的抗体，以及Western blot鉴定D. immitis的成年抗原提取物中寄生虫的特异性条带。积极的结果在GIS地图上得到了体现，其中包括与研究区域中的寄生虫传播有关的地质环境信息。犬流浪人群中D. immitis的患病率为2。12％，在1-10岁的个人中发现阳性病例。人体血清阳性率被确定为5.11％，男女的感染率相似。按年龄，年轻人群（<18岁）的血清阳性率明显高于其他人群。结果的地理位置表明，在环境中（2公里以内）发现了潜在的有利蚊子繁殖区域的阳性病例。缺乏环境卫生设施和缺乏限制或消除犬类水库人口的措施，都突出了人为因素在创造和维持城市内犬类和人铁丝虫病传播的有利条件方面的重要性。11％的男性和女性受到类似的影响。按年龄，年轻人群（<18岁）的血清阳性率明显高于其他人群。结果的地理位置表明，在环境中（2公里以内）发现了潜在的有利蚊子繁殖区域的阳性病例。缺乏环境卫生设施和缺乏限制或消除犬类水库人口的措施，都突出了人为因素在创造和维持城市内犬类和人铁丝虫病传播的有利条件方面的重要性。11％的男性和女性受到类似的影响。根据年龄，年轻人群（<18岁）的血清阳性率明显高于其他人群。结果的地理位置表明，在环境中（2公里以内）发现了潜在的有利蚊子繁殖区域的阳性病例。缺乏环境卫生设施和缺乏限制或消除犬类水库人口的措施，都突出了人为因素在创造和维持城市内犬类和人铁丝虫病传播的有利条件方面的重要性。结果的地理位置显示，在环境中（2公里内）发现了潜在的有利蚊子繁殖区域的阳性病例。缺乏环境卫生设施和缺乏限制或消除犬类水库人口的措施，都突出了人为因素在创造和维持城市内犬类和人铁丝虫病传播的有利条件方面的重要性。结果的地理位置表明，在环境中（2公里以内）发现了潜在的有利蚊子繁殖区域的阳性病例。缺乏环境卫生设施和缺乏限制或消除犬类水库人口的措施，都突出了人为因素在创造和维持城市内犬类和人铁丝虫病传播的有利条件方面的重要性。