Emerging important Acinetobacter strains commonly accommodate a plethora of mobile elements including plasmids of different size. Plasmids, apart from encoding modules enabling their self-replication and/or transmission, can carry a diverse number of genes, allowing the host cell to survive in an environment that would otherwise be lethal or restrictive for growth. The present study characterizes the plasmidome generated from an arsenic-resistant strain named ZS207, classified as Acinetobacter lwoffii. Sequencing effort revealed the presence of nine plasmids in the size between 4.3 and 38.4 kb as well as one 186.6 kb megaplasmid. All plasmids, except the megaplasmid, do apparently not confer distinguishing phenotypic features. In contrast, the megaplasmid carries arsenic and heavy metals resistance regions similar to those found in permafrost A. lwoffii strains. In-depth in silico analyses have shown a significant similarity between the regions from these plasmids, especially concerning multiple transposable elements, transfer and mobilization genes, and toxin-antitoxin systems. Since ars genes encode proteins of major significance in terms of potential use in bioremediation, arsenic resistance level of ZS207 was determined and the functionality of selected ars genes was examined. Additionally, we checked the functionality of plasmid-encoded toxin-antitoxin systems and their impact on the formation of persister cells.

中文翻译：

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源自前金和砷矿的环境不动杆菌lwoffii菌株的质粒组。

新兴的重要不动杆菌菌株通常容纳过多的移动元件，包括大小不同的质粒。质粒除了能够实现自身复制和/或传播的编码模块外，还可以携带多种基因，从而使宿主细胞能够在可能致命或限制生长的环境中存活。本研究表征了从名为ZS207的抗砷菌株产生的质粒组，该菌株被分类为不动杆菌。测序工作表明存在9个质粒，大小在4.3和38.4 kb之间，还有一个186.6 kb的大质粒。除大质粒外，所有质粒显然不具有区分表型的特征。相反，大质粒带有与永久冻土A中相似的砷和重金属抗性区域。lwoffii菌株。深入的计算机分析表明，这些质粒的区域之间存在显着相似性，特别是涉及多个转座因子，转移和动员基因以及毒素-抗毒素系统。由于ars基因编码的蛋白质在生物修复中的潜在用途方面具有重要意义，因此确定了ZS207的砷抗性水平，并检查了所选ars基因的功能。此外，我们检查了质粒编码的毒素-抗毒素系统的功能及其对持久性细胞形成的影响。由于ars基因编码的蛋白质在生物修复中的潜在用途方面具有重要意义，因此确定了ZS207的砷抗性水平，并检查了所选ars基因的功能。此外，我们检查了质粒编码的毒素-抗毒素系统的功能及其对持久性细胞形成的影响。由于ars基因编码的蛋白质在生物修复中的潜在用途方面具有重要意义，因此确定了ZS207的砷抗性水平，并检查了所选ars基因的功能。此外，我们检查了质粒编码的毒素-抗毒素系统的功能及其对持久性细胞形成的影响。