Laccase was immobilized on the electrospun chitosan/poly(vinyl alcohol) fiber (ceCPL) with specific area of 17.05 m²/g to reinforce the stability of enzymatic time–temperature indicator (TTI). The ceCPL exhibited a high relative activity within 4 °C–40 °C and the activation energy (Ea) of coloration with guaiacol approached 40.9 ± 2.8 kJ/mol for 80 μg/cm² of ceCPL-immobilized laccase. In dynamic temperature response experiments of ceCPL using intermittent temperature changes between 4 °C and 25 °C, irreversible inactivation did not occur, indicating the desirable tolerance and sensitivity of ceCPL to temperature alterations. Approximately 1 or 38 days was required to reach the endpoint of coloration at 4 °C when 80 or 8 μg/cm² of laccase were immobilized on ceCPL, respectively. The regression equation derived from kinetic model analysis was conducted and could be further utilized to design a suitable time–temperature indicator (TTI) to monitor food quality according to the color change of ceCPL coloration.

漆酶固定在比表面积为17.05 m ² / g的静电纺丝壳聚糖/聚乙烯醇纤维（ceCPL）上，以增强酶时温指示剂（TTI）的稳定性。ceCPL在4°C至40°C的温度下表现出较高的相对活性，愈创木酚酸固定化漆酶80μg / cm ²，愈创木酚的着色活化能（Ea）接近40.9±2.8 kJ / mol 。在使用4°C和25°C之间的间歇温度变化的ceCPL动态温度响应实验中，未发生不可逆的失活，这表明ceCPL对温度变化具有理想的耐受性和敏感性。当80或8μg/ cm ²时，在4°C下达到显色终点大约需要1或38天漆酶分别固定在ceCPL上。进行了从动力学模型分析得出的回归方程，可以根据ceCPL着色的颜色变化将其进一步用于设计合适的时间-温度指示器（TTI）来监控食品质量。