当前位置: X-MOL 学术Environ. MicroBiol. Rep › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Phylogenetic and structural diversity of aromatically dense pili from environmental metagenomes.
Environmental Microbiology Reports ( IF 3.3 ) Pub Date : 2020-06-01 , DOI: 10.1111/1758-2229.12848


Following the publication of the article by Bray et al. (2020), the authors alerted the Journal to the fact that, in Figure 1, the boxes for amino acids 24, 27, 32, 50, 51, and 57 are incorrectly aligned. The amended Figure is shown below. The Publisher apologises for the mistake.

image
Figure 1
Open in figure viewerPowerPoint

Basis for distinguishing e‐pilins from other pilins. Top: Alignment showing the location of aromatic residues in each pilin tested for conductivity in previous studies (Table S1). Dark horizontal lines indicate 42–53 aa aromatic‐free gaps in non‐conductive pilins. Conserved N‐terminal aromatic residues in bacterial e‐pilins are indicated by vertical boxes. All bacterial e‐pilins contained F‐1, Y‐24, Y‐27, Y/F‐51, Y/F‐50 and/or Y/F‐51, and H/Y/F‐32 and/or Y/F‐57. The only N‐terminal residues shared by archaeal and bacterial e‐pilins were F‐1 and Y‐57. Bottom: Relationship between gap size and percentage of aromatic amino acids in the mature pilin peptide for four types of pilins, which was used to establish conservative criteria for identifying putative e‐pilins in environmental metagenomes. Therefore, we used ≥98% aromatics and ≤22‐aa aromatic‐free gap (boxed area labelled ‘conductive e‐pilins’), and the presence of aromatic amino acids at residues as a conservative threshold for predicting putative e‐pilins from metagenomes, consistent with thresholds established by Walker et al. (2019a). Additional information about each pilin is in Tables S1 and S2.



中文翻译:

来自环境基因组的芳香密集菌毛的系统发育和结构多样性。

在Bray等人发表文章之后。(2020),作者提醒《华尔街日报》注意以下事实:在图1中,氨基酸24、27、32、50、51和57的框未正确对齐。修改后的图如下所示。发布者对此错误表示歉意。

图片
图1
在图形查看器中打开PowerPoint

区分e-pilins与其他pilins的基础。上图:比对显示先前研究中测试的每个菌毛素中芳香族残基的位置(表S1)。深色水平线表示非导电毛坯中的42–53 aa无芳香间隙。细菌epilins中保守的N末端芳族残基用垂直框表示。所有细菌epilins均包含F-1,Y-24,Y‐27,Y / F‐51,Y / F‐50和/或Y / F‐51,H / Y / F‐32和/或Y / F-57。古细菌和细菌epilins共有的N末端残基只有F-1和Y-57。底部:四种类型的菌毛蛋白的间隙大小与成熟菌毛蛋白肽中芳香族氨基酸的百分比之间的关系,用于建立保守的标准来鉴定环境元基因组中假定的电子菌毛蛋白。因此,我们使用了≥98%的芳族化合物和≤22-aa的无芳族化合物间隙(标有“导电性电子菌毛”的方框区域),并且残基中存在芳香族氨基酸作为从元基因组预测推定的电子菌毛素的保守阈值,与Walker等人建立的阈值一致。(2019a)。有关每个菌毛蛋白的其他信息,请参见表S1和S2。

更新日期:2020-05-03
down
wechat
bug