Plasma–liquid interactions play an important role in plasma‐mediated bacterial inactivation. Attenuated total reflectance Fourier‐transform infrared (FT‐IR) spectroscopy is employed to probe the molecular changes occurring in Escherichia coli suspension during the direct plasma treatment and subsequent after‐charge storage. The recorded spectra are correlated to the germicidal effect. Successive changes in protein secondary structures and DNA conformation are observed. It is found that spectral range around 1,345 cm⁻¹ is most susceptible to plasma. With respect to after‐charge storage, it is shown that the cell debris is mainly composed of DNA/RNA and nitric acids with fewer proteins, and the leakage of cell inclusions becomes more severe with longer plasma treatment time. Morphology alteration revealed by transmission electron microscopy suggests cell membrane injuries and cell leakage, consistent with the FT‐IR results. All these findings indicate that cell membrane damage may be the principal cause of plasma‐induced inactivation.

中文翻译：

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使用ATR FT-IR通过大气DBD血浆发现盐水中大肠杆菌的失活动力学

血浆-液体相互作用在血浆介导的细菌失活中起重要作用。衰减全反射傅立叶变换红外（FT-IR）光谱用于探测直接血浆处理和随后的电荷储存过程中大肠杆菌悬浮液中发生的分子变化。记录的光谱与杀菌效果相关。观察到蛋白质二级结构和DNA构象的连续变化。发现光谱范围约为1,345 cm ^-1对血浆最敏感。关于充电后的存储，已表明细胞碎片主要由DNA / RNA和硝酸组成，蛋白质较少，并且随着血浆处理时间的延长，细胞内含物的泄漏变得更加严重。透射电子显微镜显示的形态变化表明细胞膜损伤和细胞渗漏，与FT-IR结果一致。所有这些发现表明，细胞膜损伤可能是血浆诱导的失活的主要原因。