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Inactivation of the Ventral Pallidum by GABAA Receptor Agonist Promotes Non-rapid Eye Movement Sleep in Rats.
Neurochemical Research ( IF 4.4 ) Pub Date : 2020-05-04 , DOI: 10.1007/s11064-020-03040-z
Xuefen Zhang 1 , Ying Liu 1 , Bo Yang 1 , Haibo Xu 1
Affiliation  

GABA, the most abundant inhibitory neurotransmitter in the brain, is closely linked with sleep and wakefulness. As the largest area input to the ventral pallidum (VP), the nucleus accumbens (NAc) has been confirmed to play a pivotal role in promoting non-rapid eye movement (NREM) sleep through inhibitory projections from NAc adenosine A2A receptor-expressing neurons to VP GABAergic neurons which mostly express GABAA receptors. Although these studies demonstrate the possible role of VP GABAergic neurons in sleep-wake regulation, whether and how its modulate sleep-wake cycle is not completely clear. In our study, pharmacological manipulations were implemented in freely moving rats and then the EEG and the EMG were recorded to monitor the sleep-wake states. We found that microinjection of muscimol, a GABAA receptor agonist, into the VP increased NREM sleep in both light and dark period. Microinjection of bicuculline, a GABAA receptor antagonist, into the VP increased wakefulness in the light period. Collectively, our data identify the important role of VP GABAA receptor-expressing neurons in NREM sleep of rats which may help improve the understanding of the pathological sleep disorders.

中文翻译:

GABAA受体激动剂灭活腹侧苍白质可促进大鼠快速眼动睡眠。

GABA是大脑中最丰富的抑制性神经递质,与睡眠和清醒密切相关。作为腹侧苍白球(VP)的最大输入区域,伏隔核(NAc)已被证实通过表达NAc腺苷A2A受体的神经元抑制投射而在促进非快速眼动(NREM)睡眠中起关键作用。 VP GABA能神经元,主要表达GABAA受体。尽管这些研究证明了VP GABA能神经元在睡眠-觉醒调节中的可能作用,但其是否以及如何调节睡眠-觉醒周期尚不完全清楚。在我们的研究中,在自由活动的大鼠中进行了药理操作,然后记录了EEG和EMG以监测睡眠-觉醒状态。我们发现微注射剂muscimol(一种GABAA受体激动剂)进入VP可以增加在明亮和黑暗时期的NREM睡眠。在VP期间向VP中微注射Bicuculline(一种GABAA受体拮抗剂)可增加清醒度。总的来说,我们的数据确定了表达VP GABA A受体的神经元在大鼠NREM睡眠中的重要作用,这可能有助于增进对病理性睡眠障碍的理解。
更新日期:2020-05-04
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