Abstract In this study, we have investigated the impact of superparamagnetic magnetite nanoparticle (Fe3O4) inclusion on the chitin polysaccharide structure, together with its surface chemistry influence on the adsorption of lysozyme (LYZ). Magnetic nanoparticles (MNPs) as fillers not only endow the chitin host structure with their physic and chemical properties but also is a straightforward tool to modify or template its porous structure. Indeed, scanning electron microscopy and Brunauer–Emmett–Teller surface area measurements confirm the template effect of the MNPs on chitin. Their incorporation reduced the thickness of the pore wall and increased the surface area from chitin (34.5 m2/g) to the chitin@Fe3O4 composites (210.8 m2/g). MNPs provide the composite system an intrinsic magnetic moment that enables the magnetic recovery of the adsorbent after LYZ uptake. To characterize the magnetic composite's interaction with LYZ, the effect of pH on the absorptive capacities and kinetic parameters was examined. The results indicated that the nanocomposite presents an adsorption capacity of 488 mg/g of lysozyme at pH 9, being able to recover LYZ without diluting or pretreating the hen egg-white, leading to a 75% global yield and a purity degree >99% in only one chromatographic step.

中文翻译：

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用于溶菌酶纯化的具有磁性纳米填料的增强型几丁质凝胶

摘要 在本研究中，我们研究了超顺磁性磁铁矿纳米颗粒 (Fe3O4) 包裹体对几丁质多糖结构的影响，以及其表面化学对溶菌酶 (LYZ) 吸附的影响。作为填料的磁性纳米粒子 (MNP) 不仅赋予几丁质主体结构以物理和化学性质，而且还是修饰或模板化其多孔结构的直接工具。事实上，扫描电子显微镜和 Brunauer-Emmett-Teller 表面积测量证实了 MNP 对几丁质的模板效应。它们的加入降低了孔壁的厚度，并增加了从几丁质 (34.5 m2/g) 到几丁质@Fe3O4 复合材料 (210.8 m2/g) 的表面积。MNPs 为复合系统提供了一个固有的磁矩，使吸附剂在 LYZ 吸收后能够磁力恢复。为了表征磁性复合材料与 LYZ 的相互作用，研究了 pH 值对吸收能力和动力学参数的影响。结果表明，该纳米复合材料在 pH 9 时对溶菌酶的吸附量为 488 mg/g，无需稀释或预处理鸡蛋清即可回收 LYZ，全球收率达 75%，纯度>99%只需一个色谱步骤。