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Characterization and antibacterial activity of the essential oil obtained from the leaves of Baccharis coridifolia DC against multiresistant strains.
Microbial Pathogenesis ( IF 3.8 ) Pub Date : 2020-05-04 , DOI: 10.1016/j.micpath.2020.104223
Priscilla Ramos Freitas 1 , Ana Carolina Justino de Araújo 1 , Cristina Rodrigues Dos Santos Barbosa 2 , Debora Feitosa Muniz 1 , Janaina Esmeraldo Rocha 1 , José Bezerra de Araújo Neto 1 , Maria Milene Costa da Silva 1 , Raimundo Luiz Silva Pereira 3 , Luiz Everson da Silva 4 , Wanderlei do Amaral 4 , Cicero Deschamps 4 , Saulo Relison Tintino 1 , Jaime Ribeiro-Filho 5 , Henrique Douglas Melo Coutinho 1
Affiliation  

Essential oils are secondary metabolites with immense pharmacological potential.These substances are abundantly produced by plants of the family Asteraceae, such as Baccharis coridifolia. Previous studies have demonstrated that this species has pharmacological properties that make it a promising source of new antibacterial agents. Therefore, the present study aimed to evaluate the antibacterial and antibiotic-modulating activity of Baccharis coridifolia essential oil against multidrug-resistant (MDR) strains. The phytochemical analysis was carried out by gas chromatography coupled to Mass Spectroscopy (GC/MS), and realized the Minimum Inhibitory Concentation (MIC) and antibiotic-modulation from the microdilution method in 96-well plates. It was revealed the presence of germacrene D (23.7%), bicyclogermacrene (17.1%), and (E)-caryophyllene (8.4%) as major components. The minimum inhibitory concentration of essential oil against strains of Pseudomonas aeruginosa (512 μg/mL) and Staphylococcus aureus (128 μg/mL) demonstrated clinically relevant antibacterial activity. In addition, the combination of subinhibitory doses of the oil with conventional antibiotics showed synergism, indicating potentiation of the antibacterial effect. In conclusion, the essential oil of Baccharis coridifolia (EOBc) presented antibacterial and antibiotic-modulating activities that place this species as a source of molecules useful in the fight against bacterial resistance.

中文翻译:

提取自Baccharis coridifolia DC叶片的精油对多重耐药菌株的表征和抗菌活性。

精油是具有巨大药理潜力的次生代谢产物,这些物质由菊科(Baccharis coridifolia)家族的植物大量生产。先前的研究表明,该物种具有药理特性,使其成为新的抗菌剂的有希望的来源。因此,本研究旨在评估Baccharis coridifolia精油对多药耐药(MDR)菌株的抗菌和抗生素调节活性。通过气相色谱-质谱联用(GC / MS)进行植物化学分析,并通过微孔稀释法在96孔板中实现了最低抑菌浓度(MIC)和抗生素调节。揭示存在胚芽戊烯D(23.7%),双环germacrene(17.1%)和(E)-石竹烯(8。4%)作为主要成分。精油对铜绿假单胞菌(512μg/ mL)和金黄色葡萄球菌(128μg/ mL)的最低抑菌浓度证明具有临床相关的抗菌活性。另外,亚抑制剂量的油与常规抗生素的组合显示出协同作用,表明增强了抗菌作用。总之,Baccharis coridifolia(EOBc)的精油具有抗菌和调节抗生素的活性,使该物种成为对抗细菌耐药性有用的分子来源。亚抑制剂量油与常规抗生素的组合显示协同作用,表明增强了抗菌作用。总之,Baccharis coridifolia(EOBc)的精油具有抗菌和调节抗生素的活性,使该物种成为对抗细菌耐药性有用的分子来源。亚抑制剂量油与常规抗生素的组合显示协同作用,表明增强了抗菌作用。总之,Baccharis coridifolia(EOBc)的精油具有抗菌和调节抗生素的活性,使该物种成为对抗细菌耐药性有用的分子来源。
更新日期:2020-05-04
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