BACKGROUND MicroRNAs are emerging as new mediators in the regulation of adipocyte physiology and have been approved to play a role in obesity. Despite several studies have focused on microRNA expression profiles and functions in different metabolic tissues, little is known about their response to nutritional interventions in white adipose tissue during obesity stages, and whether they differ in this response to weight-reduction strategy is poorly understood. Our objectives were to study the dysregulation of some miRNAs in subcutaneous inguinal white adipose tissue during weight change, expansion/reduction; in response to both a high-fat diet and switching to a normal diet feeding, and to evaluate them as potential biomarkers and therapeutic targets for early obesity management METHOD: A hundred 6-week-old male Wister rats were randomly divided into a normal diet group (N.D), a high-fat diet group (H.F.D), and a switched to a normal diet group (H.F.D/N.D). At the beginning and at intervals 2 weeks, serum lipid, hormone levels, total body fat mass, and inguinal subcutaneous white adipose tissue mass (WAT) measurements were recorded using dual-energy X-ray absorptiometry (DEXA). The expression levels of microRNAs were evaluated using real-time PCR. RESULTS Significant alterations were observed in serum glucose, lipid profile, and adipokine hormones during the early stages of obesity development. Alteration in rno-mir 30a-5p, rno-mir 133a-5p, and rno-mir 107-5p expression levels were observed at more than one time point. While rno-let-7a-5p, rno-mir 193a-5p, and rno-mir125a-5p were downregulated and rno-mir130a-5p was upregulated at all time points within 2 to 4 weeks in response to H.F.D feeding for 10 weeks. The impact of switching to normal diet has a reversed effect on lipid profile, adipokine hormone levels, and some miRNAs. The bioinformatics results have identified a novel and important pathway related to inflammatory signalling. CONCLUSION Our research demonstrated significant alterations in some adipocyte-expressed miRNAs after a short time of high caloric diet consumption. This provides further evidence of the significant role of nutrition as an epigenetic factor in regulation of lipid and glucose metabolism genes by modulating of related key miRNAs. Therefore, we suggest that miRNAs could be used as biomarkers for adiposity during diet-induced obesity. Perhaps limitation in calories intake is a way to manipulate obesity and associated metabolic disorders. Further studies are needed to fully elucidate the role of microRNAs in the development of obesity.

中文翻译：

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肥胖发生过程中皮下白色脂肪组织中一些miRNA的表达谱分析。

背景技术微小RNA正在成为调节脂肪细胞生理的新介体，并且已经被批准在肥胖症中起作用。尽管有几项研究专注于不同代谢组织中microRNA的表达特征和功能，但人们对肥胖阶段对白色脂肪组织中营养干预的反应知之甚少，并且人们对减肥策略的反应是否有所不同还知之甚少。我们的目标是研究体重改变，扩张/减少过程中，腹股沟皮下白色脂肪组织中某些miRNA的失调。作为对高脂饮食和改用正常饮食喂养的回应，并将其评估为早期肥胖管理的潜在生物标志物和治疗目标。将一百只6周大的雄性Wister大鼠随机分为正常饮食组（ND），高脂饮食组（HFD）和转换为正常饮食组（HFD / ND）。在开始和间隔2周时，使用双能X线吸收法（DEXA）记录血脂，激素水平，总脂肪量和腹股沟皮下白色脂肪组织量（WAT）。使用实时PCR评估microRNA的表达水平。结果在肥胖症发展的早期阶段，观察到血糖，脂质谱和脂肪因子的显着改变。在超过一个时间点观察到rno-mir 30a-5p，rno-mir 133a-5p和rno-mir 107-5p表达水平的变化。而rno-let-7a-5p，rno-mir 193a-5p，响应HFD喂食10周，在2-4周内的所有时间点上调了rno-mir125a-5p和rno-mir125a-5p的表达，而在2-4周的所有时间点上调了rno-mir130a-5p的表达。改用正常饮食会对血脂，脂肪因子和某些miRNA产生相反的影响。生物信息学的结果已经确定了与炎症信号有关的新颖重要途径。结论我们的研究表明，在短时间内大量摄入高热量饮食后，一些脂肪细胞表达的miRNA发生了显着变化。这进一步证明了营养作为表观遗传因子通过调节相关关键miRNA调节脂质和葡萄糖代谢基因的重要作用。因此，我们建议在饮食诱导的肥胖症中将miRNA用作肥胖的生物标志物。限制卡路里的摄入可能是控制肥胖症和相关代谢紊乱的一种方法。需要进一步的研究来充分阐明microRNA在肥胖症发展中的作用。