This article presents the results of the combined effects of RGD (arginine–glycine–aspartate) functionalization and mechanical stimulation on osteogenesis that could lead to the development of implantable robust tissue‐engineered mineralized constructs. Porous polycaprolactone/hydroxyapatite (PCL/HA) scaffolds are functionalized with RGD‐C (arginine–glycine–aspartate–cysteine) peptide. The effects of RGD functionalization are then explored on human fetal osteoblast cell adhesion, proliferation, osteogenic differentiation (alkaline phosphatase activity), extracellular matrix (ECM) production, and mineralization over 28 days. The effects of RGD functionalization followed by mechanical stimulation with a cyclic fluid shear stress of 3.93 mPa in a perfusion bioreactor are also elucidated. The tensile properties (Young's moduli and ultimate tensile strengths) of the cell‐laden scaffolds are measured at different stages of cell culture to understand how the mechanical properties of the tissue‐engineered structures evolve. RGD functionalization is shown to promote initial cell adhesion, proliferation, alkaline phosphatase (ALP) activity, and ECM production. However, it does not significantly affect mineralization and tensile properties. Mechanical stimulation after RGD functionalization is shown to further improve the ALP activity, ECM production, mineralization, and tensile properties, but not cell proliferation. The results suggest that combined RGD functionalization and mechanical stimulation of cell‐laden PCL/HA scaffolds can be used to accelerate the regeneration of robust bioengineered bone structures.

中文翻译：

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机械刺激可改善成骨细胞生成和载有成骨细胞的 RGD 功能化聚己内酯/羟基磷灰石支架的机械性能。

本文介绍了 RGD（精氨酸-甘氨酸-天冬氨酸）功能化和机械刺激对成骨的综合影响的结果，这可能导致可植入的坚固组织工程矿化结构的发展。多孔聚己内酯/羟基磷灰石 (PCL/HA) 支架用 RGD-C（精氨酸-甘氨酸-天冬氨酸-半胱氨酸）肽功能化。然后在 28 天内探索 RGD 功能化对人胎儿成骨细胞粘附、增殖、成骨分化（碱性磷酸酶活性）、细胞外基质 (ECM) 产生和矿化的影响。还阐明了 RGD 功能化后在灌注生物反应器中使用 3.93 mPa 的循环流体剪切应力进行机械刺激的效果。拉伸性能（杨氏 s 模量和极限拉伸强度）在细胞培养的不同阶段进行测量，以了解组织工程结构的机械性能如何演变。显示 RGD 功能化可促进初始细胞粘附、增殖、碱性磷酸酶 (ALP) 活性和 ECM 产生。然而，它不会显着影响矿化和拉伸性能。RGD 功能化后的机械刺激显示可进一步提高 ALP 活性、ECM 产生、矿化和拉伸特性，但不会提高细胞增殖。结果表明，RGD 功能化和机械刺激的细胞负载 PCL/HA 支架的组合可用于加速坚固的生物工程骨结构的再生。