In common wheat, genes/QTL for a number of traits including grain quality and resistance against all the three rusts were pyramided in the background of an erstwhile elite Indian wheat cv. PBW343. For this purpose, two improved lines (P1 and P2) each in the background of PBW343 were crossed; these two lines were earlier developed using marker-assisted selection (MAS). The genes carried by line “P1” included Yr70/Lr76 + Lr37/Yr17/Sr38, and the genes carried by line “P2” included Gpc-B1/Yr36+QPhs.ccsu-3A.1+QGw.ccsu-1A.3+Lr24/Sr24+Glu-A1-1/Glu-A1-2. Following foreground MAS in F₂ and F₃ and SDS-PAGE analysis in F₅, 23 lines were selected, each carrying all the genes/QTL for grain quality and resistance to three rusts in homozygous condition. Eleven of the selected lines had relatively higher grain protein content (along with one or more other traits) and were also resistant to five pathotypes of Puccinia triticina (Pt), seven pathotypes of Puccinia graminis f. sp. tritici (Pgt), and two pathotypes (78S84 and 47S103) of Puccinia striiformis f. sp. tritici (Pst) that occur in major wheat-growing areas of India. One line (CCSU-7) out of these 11 selected lines had significantly higher grain yield as well as grain protein content compared with check cv. PBW343, which should prove useful in future wheat breeding programs for improving not only the grain quality but also the durability of resistance against all three rusts.

在普通小麦中，许多品质的基因/ QTL（包括籽粒品质和对所有三种锈病的抵抗力）在古老的印度优质小麦品种的背景下呈金字塔形。PBW343。为此，在PBW343的背景中分别交叉了两条改进的线（P1和P2）。这两条线是使用标记辅助选择（MAS）进行开发的。“ P1”品系携带的基因包括Yr70 / Lr76  +  Lr37 / Yr17 / Sr38，“ P2”品系携带的基因包括Gpc-B1 / Yr36 + QPhs.ccsu-3A.1 + QGw.ccsu-1A.3 + Lr24 / Sr24 + Glu-A1-1 /Glu-A1-2。F中以下前景MAS ₂和F ₃和F中的SDS-PAGE分析₅ / QTL谷物质量和耐在纯合条件下3条锈病，选择23行，每行携带所有的基因。所选择的行的十一个具有相对较高的谷物蛋白质含量（与一个或多个其他性状一起）和也耐五个致病型小麦叶锈菌（PT），7个致病型的禾柄锈菌F。sp。小麦（Pgt）和两种条锈菌（Puccinia striiformis）的两种致病型（78S84和47S103）。sp。小麦（Pst）发生在印度主要的小麦产区。与检查cv相比，在这11个选择的品系中有一个品系（CCSU-7）具有显着更高的谷物产量和谷物蛋白含量。PBW343，在将来的小麦育种计划中应被证明是有用的，它不仅可以改善谷物品质，而且还可以提高对所有三种锈病的抵抗力。