Raman and Fourier Transform Infrared (FT-IR) spectroscopy was used for investigation of structural differences of bacterial celluloses (BC), obtained by cultivation native and immobilized cells of Gluconacetobacter sucrofermentans during static and dynamic regimes of cultivation on a molasses media. It was found that the Raman and FT-IR spectra could characterized the groups of the cellulose molecules. The culturing bacterial cellulose in the presence of results in an increase of crystalline and it increased during cultivated on a molasses media with the addition of 1.5% ethanol-75.62%. The degree of BC crystallinity increased during dynamic regime of cultivation is higher than under static regime one. The maximal BC content was observed when 0.5% ascorbic acid was added to the cultivation medium with molasses and native cells. It was found, the degree of BC crystallinity during static regime cultivation on a molasses medium with ethanol, increased significantly to 73.5%, and during dynamic regime-75.6%. So, in this study, the changes of the bacterial cellulose conformation of were revealed during bacterial cultivation in a medium containing molasses in various cultivation modes.

中文翻译：

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拉曼光谱和红外光谱研究了在糖蜜培养基上不同培养方式下来自糖蔗糖杆菌的细菌的纤维素结构差异。

拉曼光谱和傅立叶变换红外（FT-IR）光谱用于研究细菌纤维素（BC）的结构差异，细菌纤维素（BC）是通过在糖蜜培养基上静态和动态培养过程中培养糖蔗糖杆菌的天然和固定化细胞而获得的。发现拉曼光谱和FT-IR光谱可以表征纤维素分子的基团。在存在细菌的情况下培养细菌纤维素会导致结晶的增加，并且在糖蜜培养基上添加1.5％的乙醇（75.62％）的过程中，其结晶性会增加。动态培养过程中BC结晶度的增加高于静态培养条件下的BC结晶度。当将0.5％的抗坏血酸加入到具有糖蜜和天然细胞的培养基中时，观察到最大的BC含量。它被找到了，在含糖蜜的糖蜜培养基上静态培养时，BC结晶度显着增加至73.5％，而在动态培养时为75.6％。因此，在这项研究中，在含有糖蜜的培养基中以各种培养方式进行细菌培养期间，揭示了细菌纤维素构象的变化。