The polysaccharides in the primary plant cell wall are a renewable energy source for biofuel production. However, these polysaccharides are not readily available for bioconversion, and large enzyme sets are required to deconstruct them. Here, we aimed to improve the glucan conversion using recombinant hemicellulases and esterase as a treatment in exploded and sugarcane bagasses (SCB), followed by the addition of commercial CBH I to prevent its inhibition by hemicellulases products. A high secretion level of the recombinant enzymes was observed on SDS-PAGE. The highest activities were verified at a temperature and pH ranging from 40 to 55 °C and 4.5 to 6.0, respectively. The released reducing sugar analysis showed that all enzymes act better on SCB, with xylanase C (XynC) presenting the best activity (0.54 U/mg of protein). The high-performance liquid chromatography (HPLC) analysis demonstrated that 24 h of pretreatment was enough to reach maximum glucan conversion. The best synergy was achieved between XynC and CBH I on SCB, 1.4%. All results showed that the enzymes acted better on SCB, which can be related to the biomass composition and its molecular structure. The enzymatic pretreatment of SCB with XynC was essential to improve the glucan conversion by CBH I.

初级植物细胞壁中的多糖是用于生物燃料生产的可再生能源。然而，这些多糖不易用于生物转化，并且需要大量的酶来解构它们。在这里，我们旨在使用重组半纤维素酶和酯酶作为爆炸和甘蔗渣（SCB）的处理方法来改善葡聚糖的转化，然后添加商业CBH I以防止其被半纤维素酶产品抑制。在SDS-PAGE上观察到重组酶的高分泌水平。分别在40至55℃和4.5至6.0的温度和pH下验证了最高的活性。释放的还原糖分析表明，所有酶都对SCB起作用，木聚糖酶C（XynC）表现出最好的活性（0.54 U / mg蛋白质）。高效液相色谱（HPLC）分析表明，预处理24小时足以达到最大葡聚糖转化率。XynC和CBH I在SCB上达到了最佳协同效果，为1.4％。所有结果表明，这些酶对SCB的作用更好，这可能与生物量组成及其分子结构有关。用XynC酶预处理SCB对于提高CBH I的葡聚糖转化率至关重要。