Cometabolic biodegradation of 1,4-dioxane (dioxane) in the presence of primary substrates is a promising strategy for treating dioxane at environmentally relevant concentrations. Seven aqueous amendments (i.e., tetrahydrofuran (THF), butanone, acetone, 1-butanol, 2-butanol, phenol and acetate) and five gaseous amendments (i.e., C₁–C₄ alkanes and ethylene) were evaluated as the primary substrates for dioxane degradation by mixed microbial consortia. The aqueous amendments were tested in microcosm bottles and the gaseous amendments were tested in a continuous-flow membrane biofilm reactor with hollow fibers pressurized by the gaseous amendments. Ethane was found to be the most effective gaseous substrate and THF was the only aqueous substrate that promoted dioxane degradation. A diverse microbial community consisting of several putative dioxane degraders—Mycobacterium, Flavobacterium and Bradyrhizobiaceae—were enriched in the presence of ethane. This is the first study showing that ethane was the most effective substrate among the short-chain alkanes and it promoted dioxane degradation by enriching dioxane-degraders that did not harbor the well-known dioxane/tetrahydrofuran monooxygenase.

中文翻译：

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研究促进1,4-二恶烷生物降解的有前途的底物：乙烷和四氢呋喃对微生物群落的影响。

在主要底物存在下，可代谢降解1,4-二恶烷（二恶烷）是一种在环境相关浓度下处理二恶烷的有前途的策略。七水修订（即，四氢呋喃（THF），丁酮，丙酮，1-丁醇，2-丁醇，苯酚和醋酸盐）和五个气态修正（即。，C ₁ -C ₄烷烃和乙烯）被评估为混合微生物财团降解二恶烷的主要底物。在缩微瓶中测试含水改性剂，并在连续流膜生物膜反应器中测试气态改性剂，其中空心纤维被气态改性剂加压。发现乙烷是最有效的气态底物，而THF是唯一促进二恶烷降解的水性底物。一个多样的微生物群落由几个假定二恶烷的degraders-分枝杆菌，黄杆菌属和慢生根瘤菌科-在乙烷存在下富集。这是第一项研究，表明乙烷是短链烷烃中最有效的底物，并且它通过富集没有已知二恶烷/四氢呋喃单加氧酶的二恶烷降解剂来促进二恶烷降解。