Xuefu Zhuyu Decoction (XFZYD) is a traditional Chinese medicine prescription used for the clinical treatment of traumatic brain injury (TBI). The purpose of this work was to develop a sensitive and rapid UHPLC–MS/MS method to simultaneously study the pharmacokinetics of nimodipine and eight components of XFZYD, namely, amygdalin, hydroxysafflor yellow A, rutin, liquiritin, narirutin, naringin, neohesperidin and saikosaponin A, in rats with and without TBI. Multiple reaction monitoring was highly selective in the detection of nine analytes and the internal standard without obvious interference. The calibration curves displayed good linearity (r  > 0.99) over a wide concentration range. The mean absolute recoveries of the nine analytes were 85–106%, and all matrix effects were in the range 80–120%. The intra‐ and inter‐day precision and accuracy were acceptable (RSD, <15%; RE%, ±20%). The validated method was successfully applied to compare the pharmacokinetics in four experimental groups, including control rats orally administered XFZYD and TBI model rats orally administered XFZYD, XFZYD and nimodipine, or nimodipine alone. The results showed that herb–drug interactions occurred between XFZYD and nimodipine in the treatment of TBI, nimodipine affected the pharmacokinetics of XFZYD, and XFZYD affected the absorption, distribution and excretion of nimodipine in vivo.

中文翻译：

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建立灵敏，快速的UHPLC-MS / MS方法，用于同时定量大鼠血浆中的九种化合物，并在口服施用血府逐瘀汤和尼莫地平后进行比较药代动力学研究。

血府逐瘀汤（XFZYD）是用于外伤性脑损伤（TBI）临床治疗的中药处方。这项工作的目的是开发一种灵敏且快速的UHPLC-MS / MS方法，以同时研究尼莫地平和XFZYD的八种成分，即苦杏仁苷，羟基红花黄A，芦丁，液化蛋白，纳鲁丁，柚皮苷，新橙皮苷和皂苷A，有和没有TBI的大鼠。在检测9种分析物和内标物时，多反应监测具有高度的选择性，且无明显干扰。校准曲线显示出良好的线性（r > 0.99）。九种分析物的平均绝对回收率为85–106％，所有基质效应在80–120％的范围内。日内和日间的精度和准确性是可以接受的（RSD，<15％； RE％，±20％）。验证的方法已成功应用于四个实验组的药代动力学比较，包括口服XFZYD的对照组大鼠和口服XFZYD，XFZYD和尼莫地平或单独尼莫地平的TBI模型大鼠。结果表明，在TBI的治疗中，XFZYD与尼莫地平之间发生草药相互作用，尼莫地平影响XFZYD的药代动力学，XFZYD影响尼莫地平在体内的吸收，分布和排泄。