The data presented in this particular study demonstrate that the biodegradation of phenol by Chlamydomonas reinhardtii is a dynamic bioenergetic process mainly affected by the production of catechol and the presence of a growth-promoting substrate in the culture medium. The study focused on the regulation of the bioenergetic equilibrium resulting from production of catechol after phenol oxidation. Catechol was identified by HPLC-UV and HPLC-ESI-MS/MS. Growth measurements revealed that phenol is a growth-limiting substrate for microalgal cultures. The Chlamydomonas cells proceed to phenol biodegradation because they require carbon reserves for maintenance of homeostasis. In the presence of acetic acid (a growth-promoting carbon source), the amount of catechol detected in the culture medium was negligible; apparently, acetic acid provides microalgae with sufficient energy reserves to further biodegrade catechol. It has been shown that when microalgae do not have sufficient energy reserves, a significant amount of catechol is released into the culture medium. Chlamydomonas reinhardtii acts as a versatile bioenergetic machine by regulating its metabolism under each particular set of growth conditions, in order to achieve an optimal balance between growth, homeostasis maintenance and biodegradation of phenol. The novel findings of this study reveal a paradigm showing how microalgal metabolic versatility can be used in the bioremediation of the environment and in potential large-scale applications.

中文翻译：

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Reinhardtii衣藻对苯酚的生物降解作用。

该特定研究中提供的数据表明，莱茵衣藻对苯酚的生物降解是一个动态的生物能过程，主要受邻苯二酚的产生和培养基中生长促进底物的存在影响。该研究的重点是调节苯酚氧化后邻苯二酚产生的生物能平衡。通过HPLC-UV和HPLC-ESI-MS / MS鉴定邻苯二酚。生长测量表明，苯酚是微藻培养物的生长限制底物。衣藻细胞进行苯酚生物降解，因为它们需要碳储备来维持体内平衡。在乙酸（一种促进生长的碳源）存在下，培养基中检出的邻苯二酚的量可以忽略不计；显然，乙酸为微藻提供了足够的能量储备，可以进一步生物降解儿茶酚。已经表明，当微藻没有足够的能量储备时，大量的儿茶酚被释放到培养基中。Reinhardtii衣藻通过在每种特定的生长条件下调节其新陈代谢来充当多功能生物能机器，从而在苯酚的生长，体内稳态维持和生物降解之间实现最佳平衡。这项研究的新发现揭示了一种范式，该范式显示了微藻代谢的多功能性如何用于环境的生物修复和潜在的大规模应用中。大量的儿茶酚释放到培养基中。Reinhardtii衣藻通过在每种特定的生长条件下调节其新陈代谢来充当多功能生物能机器，从而在苯酚的生长，体内稳态维持和生物降解之间实现最佳平衡。这项研究的新发现揭示了一种范式，该范式显示了微藻代谢的多功能性如何用于环境的生物修复和潜在的大规模应用中。大量的儿茶酚释放到培养基中。Reinhardtii衣藻通过在每种特定的生长条件下调节其新陈代谢来充当多功能生物能机器，从而在苯酚的生长，体内稳态维持和生物降解之间实现最佳平衡。这项研究的新发现揭示了一种范式，该范式显示了微藻代谢的多功能性如何用于环境的生物修复和潜在的大规模应用中。