Abstract

Bellamya bengalensis, an edible mollusca, serves as a protein rich food source for the tribes in India. The objective of the present study was to isolate the protein fraction of the edible foot part of B. bengalensis for hydrolysis with three proteases, namely papain, pepsin, and alcalase. B. bengalensis protein isolates and hydrolysates were characterised for the functional properties like protein solubility index, emulsifying property, foaming property. The proximate composition of the protein isolate was determined along with nutritional value that included biological value, protein efficiency ratio, amino acid score, nutritional index, essential amino acid index. The molecular weight distribution of the protein isolate and the three hydrolysates were analyzed by SDS-PAGE. The hydrolysates were fractionated by ultrafiltration and the in vitro antioxidative properties were measured. The antihypertensive property of the in vitro angiotensin converting enzyme inhibitory activity of the hydrolysates was compared with the standard drug lisinopril. Thus, the results indicated that the hydrolyzed peptides had potent antioxidative and antihypertensive activity. The enzyme pepsin and papain produced partially hydrolyzed peptides suitable for use in the bakery industry while alcalase hydrolysis resulted in shorter peptides with the antihypertensive activity that may be used as a promising nutraceutical.

Graphic abstract

中文翻译：

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从 Bellamya bengalensis 酶促切除的寡肽显示出有效的抗氧化和抗高血压活性。

摘要

Bellamya bengalensis 是一种可食用的软体动物，是印度部落富含蛋白质的食物来源。本研究的目的是分离出B. bengalensis可食用足部的蛋白质部分，用于用三种蛋白酶进行水解，即木瓜蛋白酶、胃蛋白酶和碱性蛋白酶。B. 孟加拉对蛋白质分离物和水解物的蛋白质溶解度指数、乳化性、起泡性等功能特性进行了表征。确定蛋白质分离物的近似组成以及营养价值，包括生物学价值、蛋白质效率比、氨基酸评分、营养指数、必需氨基酸指数。通过SDS-PAGE分析蛋白质分离物和三种水解物的分子量分布。通过超滤分级分离水解产物并测量体外抗氧化特性。将水解产物的体外血管紧张素转化酶抑制活性的抗高血压特性与标准药物赖诺普利进行了比较。因此，结果表明，水解肽具有较强的抗氧化和抗高血压活性。酶胃蛋白酶和木瓜蛋白酶产生适用于烘焙工业的部分水解肽，而碱性酶水解产生具有抗高血压活性的较短肽，可用作有前途的营养品。

图形摘要