Loop-mediated isothermal amplification (LAMP) had been employed as a powerful tool to facilitate genetic tests for various food pathogens, as it is easy to perform. Recently, various methods of detecting the LAMP amplicon were developed. In this study, we improved two LAMP assays by combining LAMP with chromatographic flow dipstick (LFD) assays for Salmonella (targeting phoP and invA, respectively). We evaluated different labeled primer sets, then selected the optimal sets to perform the LFD assays. We compared the optimal LFD and LAMP assays with the ISO standard method. The results showed that LFD was more sensitive and quicker than LAMP. Furthermore, enrichment broths of 225 food samples were tested. The sensitivity of two LFD assays was 100%. The specificity of LFD assay targeting phoP was 99.1%, and LFD assay targeting invA was 99.5%. For the LFD assay targeting phoP, the estimate of limit of detection (LOD) 50% was 0.061 CFU/g and the estimate of LOD 95% was 0.265 CFU/g. For the LFD assay targeting invA, the estimate of LOD 50% was 0.040 CFU/g and the estimate of LOD 95% was 0.172 CFU/g. We validated this method in a primary laboratory, where we accomplished the assay only using an incubator and a heating block. It suggested that the LFD assay had the potential to become a suitable diagnostic method in field test and primary labs.

环介导的等温扩增（LAMP）已被用作一种功能强大的工具，可以轻松地对各种食品病原体进行基因测试。最近，开发了多种检测LAMP扩增子的方法。在这项研究中，我们通过将LAMP与沙门氏菌的色谱流动量油计（LFD）分析相结合（针对pho P和invA）。我们评估了不同的标记引物组，然后选择最佳的组进行LFD分析。我们将最佳的LFD和LAMP分析与ISO标准方法进行了比较。结果表明，LFD比LAMP更灵敏，更快捷。此外，测试了225个食品样品的浓缩肉汤。两种LFD检测的灵敏度为100％。针对pho P的LFD测定的特异性为99.1％，针对inv A的LFD测定的率为99.5％。对于针对pho P的LFD分析，检测限（LOD）的估计值为50.61 CFU / g，LOD 95％的估计值为0.265 CFU / g。用于LFD测定的靶向药物A，LOD 50％的估计值为0.040 CFU / g，LOD 95％的估计为0.172 CFU / g。我们在主要实验室中验证了此方法，在该实验室中，仅使用培养箱和加热块即可完成测定。这表明，LFD分析有可能成为现场测试和主要实验室中合适的诊断方法。