A total of 594 Vibrio parahaemolyticus isolates from cultivated oysters (n = 361) and estuarine water (n = 233) were examined for antimicrobial resistance (AMR) phenotype and genotype and virulence genes. Four hundred forty isolates (74.1%) exhibited resistance to at least one antimicrobial agent and 13.5% of the isolates were multidrug-resistant strains. Most of the V. parahaemolyticus isolates were resistant to erythromycin (54.2%), followed by sulfamethoxazole (34.7%) and trimethoprim (27.9%). The most common resistance genes were qnr (77.8%), strB (27.4%) and tet(A) (22.1%), whereas bla_TEM (0.8%) was rarely found. Four isolates (0.7%) from oysters (n = 2) and estuarine water (n = 2) were positive to tdh, whereas no trh-positive isolates were observed. Significantly positive associations among AMR genes were observed. The SXT elements and class 1, 2 and 3 integrons were absent in all isolates. The results indicated that V. parahaemolyticus isolates from oysters and estuarine water were potential reservoirs of resistance determinants in the environment. This increasing threat of resistant bacteria in the environment potentially affects human health. A ‘One Health’ approach involved in multidisciplinary collaborations must be implemented to effectively manage antimicrobial resistance.

中文翻译：

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从养殖牡蛎和河口水中分离的副溶血性弧菌的表型和基因型抗菌素耐药性和毒力基因的分布。

检验了从养殖牡蛎（n = 361）和河口水（n = 233）中分离出的594株副溶血弧菌的耐药性（AMR）表型，基因型和毒力基因。440株（74.1％）表现出对至少一种抗菌剂的抗药性，其中13.5％的菌株为耐多药菌株。大多数副溶血性弧菌分离株对红霉素有抗性（54.2％），其次是磺胺甲恶唑（34.7％）和甲氧苄啶（27.9％）。最常见的抗性基因是qnr（77.8％），strB（27.4％）和tet（A）（22.1％），而bla _TEM（0.8％）很少被发现。从牡蛎4株（0.7％）（Ñ = 2）和水河口（Ñ = 2）为阳性，以TDH，而没有TRH观察到阳性分离物。观察到AMR基因之间的显着正相关。在所有分离株中均不存在SXT元件和1、2和3类整合素。结果表明，来自牡蛎和河口水的副溶血弧菌分离物是环境中抗性决定子的潜在储藏库。环境中耐药细菌的这种日益增加的威胁可能会影响人类健康。必须实施涉及多学科合作的“一个健康”方法，以有效管理抗菌素耐药性。