The aim of this study was to examine whether the process of initial colonization and the formation of mature biofilm structure of foodborne bacterial pathogens Listeria monocytogenes and Salmonella typhimurium can be impeded by active essential oil components β‐caryophyllene, cinnamaldehyde and eugenol at their individual and combined effects. Among the essential oil components tested, cinnamaldehyde and eugenol at their individual effect showed >50% degradation in biofilm biomass against preformed (matured) biofilms of both the studied bacteria, whereas β‐caryophyllene failed to do so. In combination, cinnamaldehyde/eugenol blend showed synergistic antibiofilm efficacy against preformed biofilms of both the studied bacteria L. monocytogenes (FICI: 0·24) and S. typhimurium (FICI: 0·40), whereas other tested combinations showed additive antibiofilm efficacy with FICI ranged from 2·02 to 2·35. Essential oil components alone and in combination also showed much higher inhibition effect on biofilm formation at the initial stage compared to their inhibition effect on preformed biofilms. The results provide evidence that cinnamaldehyde/eugenol combination may help in designing a more potent novel, natural antibiofilm blend at sufficiently low concentrations in the food and pharmaceutical industries.

中文翻译：

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评估精油成分 β-石竹烯、肉桂醛和丁香酚单独和组合对抗单核细胞增生李斯特菌和鼠伤寒沙门氏菌的生物膜形成和预形成生物膜的抗生物膜功效

本研究的目的是检查食源性细菌病原体单核细胞增生李斯特菌和鼠伤寒沙门氏菌的初始定殖过程和成熟生物膜结构的形成过程是否会受到活性精油成分 β-石竹烯、肉桂醛和丁香酚的单独和组合的阻碍。效果。在所测试的精油成分中，肉桂醛和丁子香酚在其各自作用下对两种研究细菌的预先形成（成熟）生物膜的生物膜生物量的降解超过 50%，而 β-石竹烯则没有这样做。肉桂醛/丁子香酚混合物对所研究的单核细胞增生李斯特菌（FICI：0·24）和鼠伤寒沙门氏菌（FICI：0·40）的预形成生物膜显示出协同抗生物膜功效，而其他测试的组合显示出具有 FICI 的附加抗生物膜功效，范围从 2·02 到 2·35。与它们对预先形成的生物膜的抑制作用相比，单独和组合的精油成分在初始阶段对生物膜形成的抑制作用要高得多。结果提供的证据表明，肉桂醛/丁子香酚组合可能有助于在食品和制药行业中设计出足够低浓度的更有效的新型天然抗生物膜混合物。