Although blood cells are interesting sources for genome investigations, one of the main problems in obtaining genomic DNA from blood is the restricted amount of DNA. This obstacle can be avoided by generating Epstein-Barr virus (EBV)-induced B cell lines. This study investigates the efficiency of four different methods to generate lymphoblastoid cell lines (LCLs). Blood samples (n = 120) were obtained from donors and categorized into four groups: fresh whole blood, frozen whole blood, fresh peripheral blood mononuclear cells (PBMCs), and frozen PBMCs. The samples were followed by EBV transformation to generate LCLs. Quality control and authentication of the cells were performed using multiplex PCR and short tandem repeat (STR) analyses. Finally, we assessed the success rate and amount of time to establish the cell lines in each group. The results showed that the cells were not contaminated nor were they misidentified or cross-contaminated with other cells. The success rate of LCLs generated from the whole blood groups was lower than the PBMC groups. The freezing procedures did not have any considerable effect on the establishment of lymphoblastoid cells. These established cells have been preserved in the human and animal cell bank of the Iranian Biological Resource Center (IBRC) and are available for researchers. Due to the management and transformation of a substantial number of blood samples, we recommend that researchers freeze PBMCs for further use with high efficiency and time-saving. We suggest that whole fresh blood should be directly transformed when the volume of the blood sample is less than 0.5 ml.

中文翻译：

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从新鲜和冷冻的全血和单核细胞建立和保存淋巴母细胞系。

尽管血细胞是进行基因组研究的有趣来源，但从血液中获取基因组DNA的主要问题之一是DNA的数量有限。可以通过产生爱泼斯坦-巴尔病毒（EBV）诱导的B细胞系来避免此障碍。这项研究调查了四种不同方法生成淋巴母细胞细胞系（LCL）的效率。血液样本（n = 120）来自供体，分为四类：新鲜全血，冷冻全血，新鲜外周血单核细胞（PBMC）和冷冻PBMC。样品后进行EBV转化以产生LCL。使用多重PCR和短串联重复序列（STR）分析进行细胞的质量控制和鉴定。最后，我们评估了建立每组细胞系的成功率和时间。结果表明，这些细胞没有被污染，也没有被其他细胞误识别或交叉污染。全血组产生LCL的成功率低于PBMC组。冷冻程序对淋巴母细胞的建立没有太大影响。这些建立的细胞已保存在伊朗生物资源中心（IBRC）的人类和动物细胞库中，可供研究人员使用。由于大量血液样品的管理和转化，我们建议研究人员将PBMC冷冻以便进一步使用，以节省时间和效率。我们建议当血样的体积小于0.5 ml时，应直接转化全血。