The study investigated the possibilities of fractionation of cranberry pomace after its extraction with supercritical CO₂ (scCO₂) without and with 5% ethanol (EtOH) at previously optimized 42.4 MPa pressure and 53 °C temperature. The extract was separated into heavier (HF, 1st separator, S₁) and lighter (LF, 2nd separator, S₂) fractions by decreasing temperature in the S₁ from 0 to −−30 °C at the constant 7 MPa pressure. In case of scCO₂61–74% of extracts precipitated in S₁; when EtOH was added the content of LF at 0 and −10 °C was higher, while at −20 and −30 °C the content of HF was 2.9–3.5-fold higher. LF was remarkably stronger antioxidant than HF. The differences in triacylglycerol (TAG) and fatty acid composition were not remarkable; higher percentages of higher saturation TAGs were in HFs. Higher amount of squalene was in scCO₂/EtOH extracts; its concentration in HF was up to 12-fold (at −30 °C) higher. The amounts of carotenoids were also higher in the HF. The concentrations of phytosterols, tocopherols and volatile compounds were remarkably higher in the LFs, particularly in case of scCO₂/EtOH. The results demonstrated that different classes of lipophilic compounds present in pomace may be pre-concentrated at freezing temperatures.

这项研究研究了在事先优化的42.4 MPa压力和53°C温度下，在不添加和添加5％乙醇（EtOH）的情况下，用超临界CO ₂（scCO ₂）提取蔓越莓果渣的可能性。通过在恒定的7 MPa压力下将S _1中的温度从0降低到--30℃ ，将萃取物分离为较重的馏分（HF，第一分离器，S ₁）和较轻的馏分（LF，第二分离器，S ₂）。在使用scCO _2的情况下，有61–74％的提取物沉淀在S _1中; 当添加EtOH时，0和-10°C时LF的含量更高，而在-20和-30°C时HF的含量则高2.9-3.5倍。LF比HF具有更强的抗氧化剂。三酰基甘油（TAG）和脂肪酸组成的差异不明显；高饱和度标签的百分比更高。scCO ₂ / EtOH提取物中的角鲨烯含量较高；其在HF中的浓度高出12倍（在-30°C下）。HF中类胡萝卜素的含量也较高。LF中植物甾醇，生育酚和挥发性化合物的浓度明显较高，特别是在scCO ₂ / EtOH的情况下。结果表明存在于果渣中的不同种类的亲脂性化合物可以在冷冻温度下预先浓缩。