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Endothelial cell aging detection by means of atomic force spectroscopy.
Journal of Molecular Recognition ( IF 2.7 ) Pub Date : 2020-05-01 , DOI: 10.1002/jmr.2853
Agnieszka M Kolodziejczyk 1 , Magdalena Kucinska 1 , Aleksandra Jakubowska 1 , Paulina Sokolowska 2, 3 , Marcin Rosowski 2 , Beata Tkacz-Szczesna 2 , Piotr Komorowski 1, 2, 4 , Krzysztof Makowski 4, 5 , Bogdan Walkowiak 1, 2, 4, 5
Affiliation  

Endothelial cell aging is related to changes not only in cell phenotype, such as luminal changes, intimal and medial thickening, and increased vascular stiffness, but encompasses different cell responses to various substances including drugs or nanomaterials. In the present work, time‐ and dose‐dependent elasticity changes evoked by silver nanoparticles in endothelial cells in early (below 15) passages were analyzed. Silver nanoparticle concentrations of 3, 3.6, and 16 μg/mL were selected for elasticity measurements for long incubation (24 hours) and of 1 and 3 μg/mL for monitoring dynamic elasticity changes of 1‐, 3‐, and 6‐hour incubations. Surprisingly, a significant reduction in the cells elasticity modulus at lower number of passages exposed to silver nanoparticles used at 3 μg/mL for 24 hours was demonstrated. These results are in contrast to those obtained for endothelial cells in late (33‐43) passages that may result from cellular aging in response to nanosilver. Furthermore, for short incubation times (1 and 3 hours), SNP‐induced significant increase in the cell elasticity modulus was detected. In current work, we also attempted to answer the question whether the changes in cell elasticity were induced by the silver nanoparticles stabilized with polyvinyl pyrrolidone or by stabilizer itself. Elasticity measurements were supplemented by observations made with transmission electron microscopy and scanning electron microscopy, which confirmed the presence of silver nanoparticles inside the cells and on the cell membrane. Additionally, activation of reactive oxygen species was detected for cells exposed to SNPs for 1 and 3 hours, which was accompanied by increased cell elasticity modulus suggesting a possible mechanism of observed phenomenon.

中文翻译:

通过原子力光谱法检测内皮细胞老化。

内皮细胞老化不仅与细胞表型的变化有关,如管腔变化、内膜和中层增厚以及血管硬度增加,而且还包括细胞对各种物质(包括药物或纳米材料)的不同反应。在目前的工作中,分析了早期(低于 15 代)内皮细胞中银纳米颗粒引起的时间和剂量依赖性弹性变化。选择 3、3.6 和 16 μg/mL 的银纳米颗粒浓度进行长时间孵育(24 小时)的弹性测量,选择 1 和 3 μg/mL 的银纳米颗粒浓度来监测 1、3 和 6 小时孵育的动态弹性变化. 令人惊讶的是,证明在暴露于以 3 μg/mL 使用的银纳米粒子 24 小时的较低传代次数下,细胞弹性模量显着降低。这些结果与晚期 (33-43) 代内皮细胞的结果形成对比,后者可能是由于纳米银引起的细胞老化所致。此外,在较短的孵育时间(1 和 3 小时)内,检测到 SNP 诱导的细胞弹性模量显着增加。在目前的工作中,我们还试图回答细胞弹性的变化是由用聚乙烯吡咯烷酮稳定的银纳米颗粒还是由稳定剂本身引起的问题。用透射电子显微镜和扫描电子显微镜进行的观察补充了弹性测量,这证实了细胞内部和细胞膜上存在银纳米颗粒。此外,对于暴露于 SNP 1 和 3 小时的细胞,检测到活性氧的激活,
更新日期:2020-05-01
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