Management of seed and soil borne fungal plant pathogens using fungal species belonging to the genus Trichoderma is gaining importance. Seed coating with powder based formulations of Trichoderma is most widely adopted by the researchers and farmers as well. Delivery system that leads to good adherence of fungal propagules on seed surface, minimizing the wastage of active ingredient, sustained and timely release during treatment process is very important for effective season long protection. Chitosan-PEG (Polyethylene glycol) (Cts-PEG) blend containing Trichoderma harzianum (Th4d) (Cts-PEG-Th) spores is developed and its storage stability, persistence in soil and bio efficacy against seed and soil borne pathogens of groundnut and safflower crops is studied. The blend was stable without much changes in pH throughout the storage period. Persistence studies conducted for 3 months revealed that Cts-PEG-Th amended soil, Trichoderma got released from polymer film slowly and reached a maximum of log 8 CFUs by 30 days and there after started declining to retain log 6 CFUs at 90 days. In shelf life study, the chitosan blend was able to maintain Trichoderma counts of log 10.0 and log 10.2 over a period of 6 months at storage temperatures of 30 °C and 4 °C, respectively and the antagonistic activity unaffected against three plant pathogens viz. Macrophomina phaseolina, Fusarium oxysporumf. sp.ricini and Aspergillus niger over a period of 6 months of storage. Bio efficacy testing in germination towels and green house pot studies revealed the effectiveness of seed treatment with Cts-PEG-Th blend significantly increasing the germination and seedling vigour and reducing the diseases in groundnut (peanut) and safflower.

利用木霉属的真菌种类管理种子和土壤传播的真菌植物病原体变得越来越重要。研究人员和农民也最普遍地采用木霉粉基制剂进行种子包衣。导致真菌繁殖体在种子表面上良好附着，最小化有效成分浪费，在处理过程中持续及时释放的传递系统，对于有效的长期保护非常重要。含有哈茨木霉的壳聚糖-PEG（聚乙二醇）（Cts-PEG）共混物（Th4d）（Cts-PEG-Th）孢子被开发出来，并研究了其储存稳定性，在土壤中的持久性以及对花生和红花作物的种子和土壤传播的病原体的生物功效。在整个储存期间，共混物稳定，pH值没有太大变化。进行了3个月的持久性研究表明，改良的Cts-PEG-Th土壤，木霉菌从聚合物薄膜中缓慢释放出来，到30天时达到最高log 8 CFU，之后开始下降，到90天时仍保留log 6 CFU。在保质期研究中，壳聚糖共混物在储存温度分别为30°C和4°C的情况下，能够在6个月内分别保持木霉数分别为log 10.0和log 10.2，并且对三种植物病原体的拮抗活性不受影响。菜豆（Phosarium oxysporum f。）sp。ricini和黑曲霉在6个月的储存期内。在发芽毛巾和温室盆栽研究中进行的生物功效测试表明，使用Cts-PEG-Th共混物处理种子的有效性显着提高了发芽和幼苗活力，并减少了花生（花生）和红花的病害。