The leafhopper Psammotettix alienus causes serious economic losses by directly sucking the plant sap and by transmitting plant viruses. Here, we obtained the full-genomic sequence of a novel chuvirus, Hancheng leafhopper mivirus (HCLeV), using RNA sequencing (RNA-seq) and rapid amplification of cDNA ends (RACE). The full genome of HCLeV comprises 9,852 nucleotides (nt) and includes two open reading frames (ORFs), which encode nucleocapsid and large polymerase protein, respectively. Its genomic features are similar to that of Tacheng tick virus 5, a member of chuviruses. However, HCLeV had only 36.6-44.4% identities in amino acid sequence for polymerase and 27.1-44.2% identities for nucleocapsid protein with chuviruses and other viruses in the order Mononegavirales and Jingchuvirales. Interestingly, we found this leafhopper can host two chuvirus species with different genomic structures. The discovery of this new virus potentially adds a new species to the family Chuviridae, and its new genomic structural form indicates broader genomic diversity among the chuviruses.

中文翻译：

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叶蝉 Psammotettix Alius 宿主具有不同基因组结构的 chuvirus。

叶蝉 Psammotettix Alius 通过直接吸食植物汁液和传播植物病毒，造成严重的经济损失。在这里，我们使用 RNA 测序 (RNA-seq) 和 cDNA 末端快速扩增 (RACE) 获得了一种新型楚病毒——韩城叶蝉微病毒 (HCLeV) 的全基因组序列。HCLeV 的全基因组包含 9,852 个核苷酸 (nt)，并包括两个开放阅读框 (ORF)，分别编码核衣壳和大聚合酶蛋白。其基因组特征与楚病毒属的塔城蜱病毒5型相似。然而，HCLeV 在聚合酶的氨基酸序列中只有 36.6-44.4% 的同一性，与 chuvirus 和其他病毒的核衣壳蛋白具有 27.1-44.2% 的同一性，以单核病毒和荆楚病毒为目的。有趣的是，我们发现这种叶蝉可以携带两种具有不同基因组结构的 chuvirus 物种。这种新病毒的发现可能为楚病毒科增加一个新物种，其新的基因组结构形式表明楚病毒中更广泛的基因组多样性。