Pectin is a biodegradable polysaccharide, and it has been recently applied as a gene delivery, drug delivery, wound healing and tissue engineering agent. In this study, pectin was extracted from pomelo (Citrus maxima) peel and characterized. The extraction recovery of pectin form pomelo peel was 14.5%, and it had 72.56% degree of esterification, 1,245.56 equivalent weight, 7.82% methoxyl and 68.27% anhydrouronic acid contents. Use of pomelo pectin as a hatching enhancing agent for fish embryos and its effect on hatching enzyme 1 (ZHE1) was investigated. The pectin‐exposed zebrafish embryos (100 µg/ml) showed significantly (p < .05) higher hatching rate (96.6%) compared with untreated (control) embryos (66.6%) at 60 hpf. The mRNA expression of ZHE1 was also significantly (p < .05) elevated up to 55.6‐fold in pectin‐exposed embryos at 24 hpf. In situ hybridization results revealed remarkably strong expression of ZHE1 in pectin‐exposed embryos compared with the control group. In addition, considerably larger size of the hatching gland was observed in pectin‐exposed larvae than that of the unexposed larvae group. These results clearly indicate that pectin isolated from pomelo peel has an ability to enhance the hatching process of zebrafish embryos via upregulation of ZHE1.

中文翻译：

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从柚子皮中纯化的果胶作为鱼胚的天然孵化剂

果胶是一种可生物降解的多糖，最近已被用作基因传递，药物传递，伤口愈合和组织工程制剂。在这项研究中，将果胶从柚（提取柑橘最大值）剥离并进行表征。果皮从柚皮中的提取回收率为14.5％，酯化度为72.56％，当量为1,245.56，甲氧基为7.82％，脱水糖醛酸含量为68.27％。研究了柚果胶作为鱼胚孵化增强剂的用途及其对孵化酶1（ZHE1）的影响。暴露于果胶的斑马鱼胚胎（100 µg / ml  ）在60 hpf时的孵化率（96.6％）显着高于未处理（对照）（66.6％）的孵化率（p <.05）。ZHE1的mRNA表达也显着（p  <.05）在果胶暴露的胚胎中以24 hpf升高至55.6倍。原位杂交结果显示，与对照组相比，果胶暴露的胚胎中ZHE1的表达非常强。此外，在果胶暴露的幼虫中观察到的孵化腺比未暴露的幼虫组大得多。这些结果清楚地表明，从柚皮中分离出的果胶具有通过上调ZHE1来增强斑马鱼胚胎孵化过程的能力。