This study aimed to understand the efficacy and mechanisms of action of an aerosolized glycol‐ethanol formulations against bacteria. We validated a small‐scale in‐house test chamber to determine the microbicidal efficacy of four aerosolized formulations combining dipropylene glycol and ethanol against Staphylococcus aureus and Escherichia coli embedded in alginate. The aerosolized glycol/ethanol formulation decreased bacterial viability by 3 log10 and was more efficacious than an ethanol only control formulation. Electron microscopic examination indicated extensive structural damage in both bacteria, and membrane damage was confirmed with potassium release in S. aureus and DNA release in E. coli. The development of a small test chamber facilitated the measurement of the microbicidal efficacy and experiments to understand the mechanism of action of an aerosolized microbicidal formulation.

中文翻译：

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使用小型便携式试验室来确定雾化乙二醇制剂的杀菌功效

本研究旨在了解雾化乙二醇-乙醇制剂对细菌的功效和作用机制。我们验证了一个小规模的内部测试室，以确定四种结合二丙二醇和乙醇的雾化制剂对嵌入藻酸盐中的金黄色葡萄球菌和大肠杆菌的杀菌功效。雾化的乙二醇/乙醇制剂将细菌活力降低了 3 log10，并且比仅含乙醇的对照制剂更有效。电子显微镜检查表明两种细菌都有广泛的结构损伤，金黄色葡萄球菌中钾释放和大肠杆菌中 DNA 释放证实了膜损伤。